Abstract
Two papers have recently described techniques for stimulating cells from mouse haemopoietic tissue to proliferate in vitro in solid agar to form cell colonies. Pluznik and Sachs1,2 used feeder layers of mouse embryonic cells or cell free extracts of embryonic tissues to stimulate colony formation by spleen cells, while Bradley and Metcalf3 described similar colony formation by bone marrow cells when neonatal mouse kidney cells were used as a feeder layer. In the latter work the colonies were found to be aggregates of myelopoietic cells in various stages of differentiation, mixed with mononuclear cells containing varying amounts of metachromatic cytoplasmic granules.
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References
Pluznik, D. H., and Sachs, L., J. Cell. Comp. Physiol., 66, 319 (1965).
Pluznik, D. H., and Sachs, L., Exp. Cell. Res. (in the press).
Bradley, T. R., and Metcalf, D., Austral. J. Exp. Biol. and Med. Sci, 44, 287 (1966).
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BRADLEY, T., METCALF, D. & ROBINSON, W. Stimulation by Leukaemic Sera of Colony Formation in Solid Agar Cultures by Proliferation of Mouse Bone Marrow Cells. Nature 213, 926–927 (1967). https://doi.org/10.1038/213926a0
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DOI: https://doi.org/10.1038/213926a0
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