Abstract
THE first report on the cultivation of tick (Ixodidae) tissues in vitro was that of Řeháček1 in, Czechoslovakia, who obtained proliferation of fibroblast type cells in explants from developing adults of Dermacentor marginatus. Řeháček2,3 afterwards improved his techniques and was able to obtain cell monolayers by trypsinizing tissues of developing adults of D. marginatus, D. pictus and Hyalomma dromedarii and growing them in test-tubes and Carrol flasks. His methods for preparing tick tissues and of culturing them are fully described elsewhere4.
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References
Řeháček, J., Acta Virol., 2, 253 (1958).
Řeháček, J., Acta Virol., 6, 188 (1962).
Řeháček, J., Ann. Épiphyties., 14, 199 (1963).
Řeháček, J., J. Med. Entomol. (in the press).
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VARMA, M., WALLERS, W. An Improved Method for obtaining, in vitro, Uniform Cell Monolayer Sheets from Tissues of the Tick, Hyalomma dromedarii (Ixodidae). Nature 208, 602–603 (1965). https://doi.org/10.1038/208602a0
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DOI: https://doi.org/10.1038/208602a0
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Tissue Culture Association Manual (1979)