Abstract
So far there has been no really satisfactory method for the quantitative separation in high-molecular form of a mixture of DNA and RNA isolated from cells. The procedure based on the different solubility of DNA and RNA in salt solutions of varying concentration1–3 is quantitatively unsatisfactory. Another method uses chromatographic separation on methylated albumin4–7, but involves a series of steps which entail the danger of degradation in the recovery of the macromolecule. Specifically, single-stranded DNA cannot be quantitatively recovered without loss of its high-molecular character, because complete elution occurs only at strongly alkaline pH. Nygaard and Hall8 have recently described a micro method for the detection of DNA/RNA hybrids based on their adsorption on membrane niters of nitrocellulose containing material.
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KLAMERTH, O. Separation of High-molecular Deoxyribonucleic Acid and Ribonucleic Acid. Nature 208, 1318–1319 (1965). https://doi.org/10.1038/2081318a0
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DOI: https://doi.org/10.1038/2081318a0
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