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Effect of Phytohaemagglutinin on Synthesis of ‘Rapidly-labelled’ Ribonucleic Acid in Human Lymphocytes

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Abstract

THE treatment of human peripheral blood leucocyte cultures with phytohaemagglutinin (PHA) causes a transformation of small lymphocytes to primitive blast cells after morphological and cytochemical changes1–4. The cells undergoing the transformation acquire the ability to synthesize DNA5,6 and the morphological changes observed after 24–72 h of incubation are accompanied by an increase of RNA synthesis7,8.

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References

  1. Nowell, P. C., Cancer Res., 20, 462 (1960).

    CAS  PubMed  Google Scholar 

  2. Mackinney, jun., A. A., Stohlman, jun., F., and Brecher, G., Blood, 19, 349 (1962).

    PubMed  Google Scholar 

  3. Elves, M. W., and Wilkinson, J. F., Exp. Cell Res., 30, 200 (1963).

    Article  CAS  Google Scholar 

  4. Quaglino, D., Hayhoe, F. G. J., and Flemans, R. J., Nature, 196, 338 (1962).

    Article  ADS  CAS  Google Scholar 

  5. Bender, M. A., and Prescott, D. M., Exp. Cell Res., 27, 221 (1962).

    Article  CAS  Google Scholar 

  6. Byron, J. W., and Lajtha, L. G., Blood, 20, 102 (1962).

    Google Scholar 

  7. Epstein, L. B., and Stohlman, jun., F., Blood, 24, 69 (1964).

    CAS  PubMed  Google Scholar 

  8. Hayhoe, F. G. J., and Quaglino, D., Nature, 205, 151 (1965).

    Article  ADS  CAS  Google Scholar 

  9. Gude, W. D., Upton, A. C., and Odell, T. T., J. Stain Techn., 30, 161 (1955).

    Article  CAS  Google Scholar 

  10. Jacob, F., and Monod, J., J. Molec. Biol., 3, 318 (1961).

    Article  CAS  Google Scholar 

  11. Hirschorn, K., Bach, F., and Kolody, R., Science, 142, 1185 (1963).

    Article  ADS  Google Scholar 

  12. Weiss, S. B., Proc. U.S. Nat. Acad. Sci., 46, 1020 (1960).

    Article  ADS  CAS  Google Scholar 

  13. Hirschorn, K., and Kolody, R., Nature, 201, 15 (1964).

    Google Scholar 

  14. Michałowski, A., Jasińska, J., Brzosko, W. J., and Nowosławski, A., Exp. Cell Res., 34, 417 (1964).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Histology and Embryology, Medical School, Warsaw, Poland

    ZBIGNIEW DARZYNKIEWICZ, TADEUSZ KRASSOWSKI & EWA SKOPIŃSKA

Authors
  1. ZBIGNIEW DARZYNKIEWICZ
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  2. TADEUSZ KRASSOWSKI
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  3. EWA SKOPIŃSKA
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DARZYNKIEWICZ, Z., KRASSOWSKI, T. & SKOPIŃSKA, E. Effect of Phytohaemagglutinin on Synthesis of ‘Rapidly-labelled’ Ribonucleic Acid in Human Lymphocytes. Nature 207, 1402–1403 (1965). https://doi.org/10.1038/2071402a0

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