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Labelled Cytoplasmic Organelles in Allium cepa Var. ‘White Lisbon’ after Administration of Tritiated Thymidine

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Abstract

THE specificity of tritium- or carbon-14-labelled thymidine as a precursor for deoxyribonucleie acid (DNA) appears to be good in many organisms1,2. Autoradiographs examined by light and electron microscopes have demonstrated that most of the label is incorporated into nuclear chromatin during the DNA synthetic phase of cell reproduction or during an exchange process in differentiated cells3–5. Occasional evidence for the cytoplasmic incorporation of thymidine has been obtained with animal cells after treatment with deoxyribonuclease6. In egg cells and developing embryos of vertebrates there is biochemical evidence for the presence of extranuclear DNA7–9.

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Authors and Affiliations

  1. Biology Department, Royal Free Hospital School of Medicine, 8 Hunter Street, London, W.C.1

    G. C. BUDD & G. M. MILLS

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  1. G. C. BUDD
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  2. G. M. MILLS
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BUDD, G., MILLS, G. Labelled Cytoplasmic Organelles in Allium cepa Var. ‘White Lisbon’ after Administration of Tritiated Thymidine. Nature 205, 524–525 (1965). https://doi.org/10.1038/205524a0

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