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Antigenic Components in Human Pituitary Follicle-stimulating Hormone Preparations of Various Purities

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Abstract

THE production of an antiserum to a preparation of human pituitary follicle-stimulating hormone (HP-FSH) which had been fractionated by electrophoresis on starch gel (SG4) has been described previously1,2. Immunoelectrophoresis of various HP-FSH preparations with this antiserum showed the presence of two precipitin lines. These two lines obtained with antigens of 1 mg/ml. solutions (w/v) were now investigated by comparing three HP-FSH preparations of different purity, CM 1 (ref. 3), CP 1 (ref. 3) and SG 4 (Fig. 1, A, B, C), and by absorbing the antiserum with various proteins.

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References

  1. Butt, W. R., Crooke, A. C., Cunningham, F. J., and Wolf, Annelisese, J. Endocrinol., 25, 541 (1963).

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  2. Wolf, Anneliese, Nature, 198, 1308 (1963).

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  3. Butt, W. R., Crooke, A. C., and Cunningham, F. J., Biochem. J., 81, 596 (1961).

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  4. Hartree, Anne S., Butt, W. R., and Kirkham, K. E., J. Endocrin., 29, 61 (1964).

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WOLF, A. Antigenic Components in Human Pituitary Follicle-stimulating Hormone Preparations of Various Purities. Nature 205, 504–505 (1965). https://doi.org/10.1038/205504a0

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