Skip to main content
SpringerLink
Log in

Changes in the Constitution of Myelin caused by Ions

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

IN an investigation of conditions which produce in vitro changes of myelin similar to those which occur in Wallerian degeneration (with formation of ovoids and spheres) the importance of the presence of a sufficient amount of calcium in the medium became apparent. A similar observation was made years ago by Nageotte1. The effects of high concentrations of calcium or of sodium ions on the constitution of myelin were therefore examined. Central and peripheral myelins homogenized with equal amounts of either 1 M sodium chloride or 0.5 M calcium chloride were extracted by water or by chloroform/methanol (2 : 1) and the extracts and residues were chemically analysed. Comparable experiments were performed by histochemical means on teased and cryostat-sectioned fresh central and peripheral nervous tissue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nageotte, J., C.R. Acad. Sci., Paris, 150, 731 (1910).

    Google Scholar 

  2. Clowes, G. H. A., J. Phys. Chem., 20, 407 (1916).

    Article  CAS  Google Scholar 

  3. Leathes, J. B., The Lancet, 208, 957 (1925).

    Article  Google Scholar 

  4. Dervichian, D. G., Trans. Faraday Soc., 42B, 180 (1947).

    Article  Google Scholar 

  5. Luzzati, V., and Husson, F., J. Cell Biol., 12, 207 (1962).

    Article  CAS  Google Scholar 

  6. Stoeckenius, W., J. Cell Biol., 12, 221 (1962).

    Article  CAS  Google Scholar 

  7. Dourmashkin, R. B., Dougherty, M., and Harris, R. J. C., Nature, 194, 1116 (1962).

    Article  ADS  CAS  Google Scholar 

  8. Husson, F., and Luzzati, V., Nature, 197, 822 (1963).

    Article  ADS  CAS  Google Scholar 

  9. Beaufay, H., and De Duve, C., Biochem. J., 73, 604 (1959).

    Article  CAS  Google Scholar 

  10. Conchie, J., and Levvy, G. A., in Methods of Separation of Subcellular Structural Components, edit. by Grant, J. K. (Biochem. Soc. Symp. No. 23, Cambridge, 1963).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Government Hospital, Tel-Hashomer, Israel

    M. WOLMAN & H. WIENER

Authors
  1. M. WOLMAN
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. WIENER
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

WOLMAN, M., WIENER, H. Changes in the Constitution of Myelin caused by Ions. Nature 200, 886–887 (1963). https://doi.org/10.1038/200886a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/200886a0

  • Springer Nature Limited

This article is cited by

Search

Navigation