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Separation of a Bacterial Proteinase on Fritted Glass Filters

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Abstract

THE proteinase of Bacillus larvae contains two components separable by paper chromatography in ascending phosphate buffer flow1,2. Preparative separation of the components was achieved with differential adsorption of the complex on cellulose powder and activated alumina. The chromatographiecally mobile component (I) was isolated following adsorption of the immobile component (II) on cellulose. Complete removal of II required several successive treatments with cellulose. The proteinase-containing extract could not be clarified by fritted glass filters without alteration of the properties of II.

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References

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  5. Minnesota Mining and Manufacturing Co., St. Paul, Minnesota.

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Author notes

  1. T. A. GOCHNAUER

    Present address: Entomology Research Institute, Central Experimental Farm, Canada Department of Agriculture, Ottawa

Authors and Affiliations

  1. Department of Entomology, Fisheries, and Wildlife, University of Minnesota, St. Paul, 1

    T. A. GOCHNAUER & H. Y. YAMAMOTO

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  1. T. A. GOCHNAUER
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  2. H. Y. YAMAMOTO
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GOCHNAUER, T., YAMAMOTO, H. Separation of a Bacterial Proteinase on Fritted Glass Filters. Nature 198, 1226–1227 (1963). https://doi.org/10.1038/1981226a0

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