Abstract
IN the nineteenth century Fodéra1 used ferrous sulphate, converted to prussian blue, to demonstrate lymphatic vascular absorption in the experimental animal. With the introduction of pharmaceutical iron colloids, Turner-Warwick2 found that ‘Inferon’ Benger (iron-oxide dextran complex) could similarly be used to demonstrate the afferent lymphatic vessels of the living female breast. For cleared specimens the staining method had certain disadvantages chiefly due to the solubility of prussian blue in alcohol during dehydration3.
Similar content being viewed by others
References
Fodéra, M., Recherches Expérimentale sur l'absorption et l'exhalation (Ballière, Paris, 1924).
Turner-Warwick, R. T., Lancet, 1371 (1955).
Turner-Warwick, R. T., Ann. Roy. Coll. Surg. Eng., 24, 101 (1959).
Hudack, S., and McMaster, P. D., J. Exp. Med., 56, 223 (1932).
McMaster, P. D., The Harvey Lectures, 37, 227 (1941–42).
Florey, H. W., and Pullinger, B. D., Brit. J. Exp. Path., 16, 49 (1935).
Hudack, and McMaster, P. D., J. Exp. Med., 57, 751 (1933).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WELLS, F. An Improved Intravital Dye Iron-Prussian Blue Method for the in vitro Examination of Living Lymphatic Vessels. Nature 195, 188–189 (1962). https://doi.org/10.1038/195188a0
Issue Date:
DOI: https://doi.org/10.1038/195188a0
- Springer Nature Limited