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An Improved Intravital Dye Iron-Prussian Blue Method for the in vitro Examination of Living Lymphatic Vessels

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Abstract

IN the nineteenth century Fodéra1 used ferrous sulphate, converted to prussian blue, to demonstrate lymphatic vascular absorption in the experimental animal. With the introduction of pharmaceutical iron colloids, Turner-Warwick2 found that ‘Inferon’ Benger (iron-oxide dextran complex) could similarly be used to demonstrate the afferent lymphatic vessels of the living female breast. For cleared specimens the staining method had certain disadvantages chiefly due to the solubility of prussian blue in alcohol during dehydration3.

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References

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WELLS, F. An Improved Intravital Dye Iron-Prussian Blue Method for the in vitro Examination of Living Lymphatic Vessels. Nature 195, 188–189 (1962). https://doi.org/10.1038/195188a0

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