N-Terminal Amino-Acids of Bovine Antibody

Abstract

BOVINE γ-globulin has been shown to be a mixture of molecules having N-terminal amino-acids, aspartic acid, glutamic acid, serine, alanine and valine with a total of less than 1 mole/mole of γ-globulin¹. A partial fractionation was achieved by partition chromatography of γ-globulin ; the molecules with N-terminal aspartic acid, serine and alanine were concentrated in the faster running fractions, but valine, the principal N-terminal acid, was present in all the fractions². Glutamic acid was present in only trace amounts in all the fractions.