A Synergic Method of carrying out Continuous Electrochromatography

Abstract

ALTHOUGH some workers using the technique of continuous electrochromatography on conventional anticonvection media (such as filter paper or glass powder) have supposed that specific adsorption effects play a part in the separation, the extent to which these can in fact influence the separation, if at all, is negligible. The adsorbed Stern layer ions are practically immobile with respect to both electrophoretic and hydrodynamic gradients; thus even notionally, since each is equally affected, the resultant of motion in the two mutually perpendicular directions should be independent of the degree of adsorption. Weber¹ has considered the general differential equation describing the concentration distribution (as a function of space and time) in terms of diffusion, adsorption, and the two velocities, on the assumption that adsorption equilibrium is reached instantaneously. The solution of this equation for the stationary state does not involve an adsorption term.