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Visualization of Acid and Alkaline Phosphatase after Starch-Gel Electrophoresis of Seminal Plasma, Serum and Bile

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Abstract

BY starch-gel electrophoresis1 the acid phosphatase of seminal plasma can be confined to a narrow zone, apparently separated from other proteins in semen2. If this technique of separation is to be used for preparative purpose it is, however, necessary to be able to localize the fraction containing phosphatase without disrupting or destroying the gel. The following is a preliminary report on the successful use of a test-paper technique for the visualization of the acid phosphatase fraction. The same method was also used to localize the fractions containing alkaline phosphatase activity after starch-gel electrophoresis of serum and bile.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Karolinska Sjukhuset, Stockholm, 60, Sweden

    BENGT ESTBORN

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  1. BENGT ESTBORN
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ESTBORN, B. Visualization of Acid and Alkaline Phosphatase after Starch-Gel Electrophoresis of Seminal Plasma, Serum and Bile. Nature 184, 1636–1637 (1959). https://doi.org/10.1038/1841636a0

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