Abstract
WHEN a monolayer of chick fibroblasts is exposed to infection with a suitable dilution of Western Equine Encephalomyelitis virus and afterwards incubated under a layer of agar containing cell nutrients, areas of cell destruction or ‘plaques’ are produced; these were shown by Dulbecco1 to result from infection by single virus particles. The plaque technique has since been applied to a number of other viruses; but the presence of cell destruction or a cytopathic effect is normally considered to be a prerequisite for the success of the technique.
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References
Dulbecco, R., Proc. U.S. Nat. Acad. Sci., 38, 747 (1952).
Kissling, R. E., Proc. Soc. Exp. Biol. N.Y., 96, 290 (1957).
Bhatt, P. V., and Work, T. H., Proc. Soc. Exp. Biol. N.Y., 96, 213 (1957).
Porterfield, J. S. (unpublished results).
Theiler, M., and Smith, H. H., J. Exp. Med., 65, 767 (1937).
Gey, G. O., and Gey, M. K., Amer. J. Cancer, 27, 45 (1936).
Trans. Roy. Soc. Trop. Med. and Hyg. (to be published).
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PORTERFIELD, J. Plaque Production with Yellow Fever and Related Arthropod-borne Viruses. Nature 183, 1069–1070 (1959). https://doi.org/10.1038/1831069b0
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DOI: https://doi.org/10.1038/1831069b0
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