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Morphological Changes of Tubercle Bacilli caused by Staining Procedures

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Abstract

WE have studied with the electron microscope the morphological changes of tubercle bacilli caused by Ziehl-Neelsen's and Fontes' stainings. BCG was stained on the specimen-holders by these methods and photographed with the light microscope, using the volatile anisol immersion-system; the same bacilli were photographed again with the electron microscope. A similar method has been used by Takeya et al. 1 and Glauert and Brieger2 in other staining procedures.

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References

  1. Takeya, K., Koike, M., Uchida, T., Inoue, S., and Nomiyama, K., J. Electron Micr., 2, 29 (1954).

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  2. Glauert, A. M., and Brieger, E. M., J. Gen. Microbiol., 13, 310 (1955).

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Authors and Affiliations

  1. Research Institute for Tuberculosis and Leprosy, Tôhoku University, Sendai

    T. EBINA, C. SHINOHARA, H. SAITO, K. FUKUSHI & J. SUZUKI

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  1. T. EBINA
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  2. C. SHINOHARA
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  3. H. SAITO
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  4. K. FUKUSHI
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  5. J. SUZUKI
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EBINA, T., SHINOHARA, C., SAITO, H. et al. Morphological Changes of Tubercle Bacilli caused by Staining Procedures. Nature 180, 42–43 (1957). https://doi.org/10.1038/180042a0

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