Skip to main content
SpringerLink
Log in

Protein Metabolism, Respiration and Growth: A Synthesis of Results from the use of 14C-Labelled Substrates and Tissue Cultures

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. The presence of Prof. E. W. Yemm, of the University of Bristol, England, as a Visiting Rockefeller Fellow in the Department of Botany at Cornell University provided the occasion for a re-investigation of the use of glutamine and γ-aminobutyric acid by plants and especially by carrot tissue cultures. The facilities for the experiments employing tissue cultures, with and without coconut milk, under the standardized conditions which are required were already well established in this Department and, with the assistance of Miss K. Mears, these facilities were being used in a variety of investigations supported by the National Cancer Institute of the U.S. Department of Health, Education and Welfare. At the planning stage the role of glutamine had been discussed by one of us (F. C. S.) with Prof. G. Krotkov, of the Department of Biology, Queen's University, Kingston, Ontario, and to his interest in the problem we owe the supply of the 14C-labelled substrates which were used. One of us (R. G. S. B.) from the laboratory at Queen's University undertook the responsibility for the radioactive measurements, leading to the specific activity determinations of the various metabolites studied. The necessary chromatographic separations and the use of quantitative paper chromatography and radioautography were carried out in the Department of Botany at Cornell University, and in this work we wish to acknowledge the help of Dr. J. K. Pollard, Mr.David Margolis, and Mrs. Greenwood, all working with one of us (F. C. S.).

  2. Caplin, S. M., and Steward, F. C., Nature, 163, 920 (1949).

    Article  ADS  Google Scholar 

  3. Steward, F. C., Caplin, S. M., and Millar, F. V., Ann. Bot. (N.S.), 16, 57 (1952).

    Article  CAS  Google Scholar 

  4. Steward, F. C., and Caplin, S. M., Int. Union Biol. Sci., Ser. B, No. 20, 385 (1955).

  5. Shantz, E. M., and Steward, F. C., J. Amer. Chem. Soc., 74, 6133 (1952).

    Article  CAS  Google Scholar 

  6. Steward, F. C., and Shantz, E. M., Int. Union Biol. Sci., Ser. B, No. 20, 399 (1955).

  7. Shantz, E. M., and Steward, F. C., J. Amer. Chem. Soc., 77, 6351 (1955).

    Article  CAS  Google Scholar 

  8. Steward, F. C., and Shantz, E. M., Proc. Wye Conf. on Growth Substances, ed. Wain and Wightman (1955).

    Google Scholar 

  9. Miller, C. O., Skoog, F. S., Okimura, M. H., Van Saltza and Strong, F. M., J. Amer. Chem. Soc., 77, 2662 (1955).

    Article  CAS  Google Scholar 

  10. Steward, F. C., Thompson, J. F., and Dent, C. E., Science, 110, 439 (1949).

    Google Scholar 

  11. Steward, F. C., and Pollard, J. F., “Inorganic Nitrogen Metabolism” (Johns Hopkins Press, Baltimore, 1956).

    Google Scholar 

  12. Gregory, F. G., and Sen, P. K., Ann. Bot., N.S., 1, 521 (1937).

    Article  CAS  Google Scholar 

  13. Steward, F. C., Stout, P. R., and Preston, C., Plant Physiol., 15, 409 (1940).

    Article  CAS  Google Scholar 

  14. Steward, F. C., and Street, H. E., “Ann. Rev. Biochem.”, 16, 471 (1947).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. R. G. S. BIDWELL

    Present address: Atlantic Regional Laboratory of the National Research Council of Canada, Halifax, Nova Scotia

Authors and Affiliations

  1. Department of Botany, Cornell University, Ithaca, N.Y.

    F. C. STEWARD

  2. Department of Biology, Queen's University, Kingston, Ontario

    R. G. S. BIDWELL

  3. Department of Botany, University of Bristol,

    E. W. YEMM

Authors
  1. F. C. STEWARD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. G. S. BIDWELL
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. W. YEMM
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

STEWARD, F., BIDWELL, R. & YEMM, E. Protein Metabolism, Respiration and Growth: A Synthesis of Results from the use of 14C-Labelled Substrates and Tissue Cultures. Nature 178, 734–738 (1956). https://doi.org/10.1038/178734a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/178734a0

  • Springer Nature Limited

This article is cited by

Search

Navigation