Abstract
DIANZANI1 has shown that ditetrazolium can be used for demonstrating the activity of, among other enzymes, tyramine (amine) oxidase in mitochondria isolated from liver and kidney. The essential reaction here is a dehydrogenation2, and hydrogen acceptors other than oxygen may be used in the oxidation of tyramine by amine oxidase3. It is therefore of interest that amine oxidase activity can be demonstrated in frozen sections of the tissue, using a tetrazolium compound as the hydrogen acceptor; even though the method is not entirely satisfactory, it shows the general distribution of the enzyme. Neotetrazolium4 was found to be much more satisfactory than blue (di-)tetrazolium.
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References
Dianzani, M. U., Nature, 171, 125 (1953).
Richter, D., Biochem. J., 31, 2022 (1937).
Philpot, F. J., Biochem. J., 31, 856 (1937).
Shelton, E., and Schneider, W. C., Anat. Rec., 112, 61 (1952).
Blaschko, H., Richter, D., and Schlossmann, H., Biochem. J., 31, 2187 (1937).
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FRANCIS, C. Histochemical Demonstration of Amine Oxidase in Liver. Nature 171, 701–702 (1953). https://doi.org/10.1038/171701b0
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DOI: https://doi.org/10.1038/171701b0
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