Abstract
IN filter-paper partition chromatography, the ninhydrin reaction has been used almost exclusively for the identification and quantitative assay of amino-acids. One of the chief disadvantages of the blue pigment formed in this reaction is its rapid fading on filter-paper chromatograms. This makes it difficult to construct permanent or semi-permanent reference maps from which not only the type of amino-acid, but also the approximate quantity of the unknown mixture, can be deduced.
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References
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KAWERAU, E., WIELAND, T. Conservation of Amino-acid Chromatograms. Nature 168, 77–78 (1951). https://doi.org/10.1038/168077b0
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DOI: https://doi.org/10.1038/168077b0
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