A New Method for the Preparation of Adsorbents for Chromatography

Abstract

THE technique of chromatography, as used at present, is limited to a few adsorbents and usually to non-aqueous systems, due to the difficulty of elution of the adsorbate. However, aqueous systems could be used if adsorbents were available which permitted adsorption and desorption to be controlled by pH. adjustment. This procedure is possible in some cases with synthetic ion exchange resins which have been used to a limited extent for the separation of mixtures of inorganic ions, organic bases such as thiamine, and some amino-acids. However, organic bases cannot always be recovered quantitatively even by elution with strong acids. ‘Sodium cotton succinate' has been used for the quantitative adsorption and recovery of histamine, which could not be eluted quantitatively from ion exchange resins¹.