Displacement Chromatography on Cation-Exchange Materials

Abstract

In recent years there have been many attempts to utilize the properties of the newer synthetic ion-exchange reagents for the separation of bases and amino-acids, but the application of truly chromato-graphic procedures has met with only partial success. In the course of the present investigation, it became clear that very sharp separations could be obtained on cation-exchange columns by the utilization of a strong base to displace others in the system. It was found that such a procedure could be reduced to the terms of the ‘displacement development’ principle introduced by Tiselius¹ for separations on adsorption columns of carbon or alumina, and for this reason we have made use of the theoretical background developed by this worker and his colleagues.