Abstract
Black rot is a bacterial disease of Brassica oleracea caused by Xanthomonas campestris pv. campestris. Resistance to the major black rot races 1 or 4 has been identified in related Brassica species including B. carinata and B. napus. In this study, two B. juncea accessions (A 19182 and A 19183) that are resistant to races 1 and 4 of Xcc were used as maternal and paternal parents to generate interspecific hybrids with B. oleracea cultivars. Interspecific hybrids were recovered using the embryo rescue technique and confirmed through inheritance of paternal molecular markers. Twenty-six interspecific hybrid plants were obtained between A 19182 and B. oleracea cultivars, but no interspecific hybrids were obtained using A 19183. Although interspecific hybrid plants were male sterile, they were used successfully as maternal parents to generate backcross plants using embryo rescue. All hybrid and BC1 plants were resistant to black rot races 1 and 4.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ayotte, R., P. M. Harney & V. Souza Machado, 1987. The transfer of triazine resistance from Brassica napus L. to B. oleracea L. I. Production of F1 hybrids through embryo rescue. Euphytica 36: 615–624.
Bain, D. C., 1952. Reaction of Brassica seedlings to black rot. Phy-topathology 42: 497–500.
Boodley, J. W. & R. Sheldrake Jr, 1982. Cornell peat-lite mixes for commercial plant growing. NY Agric Exp Sta Agric Inf Bull 43.
Camargo, L. E. A., P. H. Williams & T. C. Osborn, 1995. Mapping of quantitative loci controlling resistance to Brassica oleracea to Xanthomonas campestris pv. campestris in the field and green-house. Phytopathology 85: 1296–1300.
Chiang, M. S., B. Y. Chiang & W. F. Grant, 1977. Transfer of resis-tance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var. 'capitata'). I. Interspecific hybridiza-tion between B. napus and B. oleracea var. 'capitata'. Euphytica 26: 319–326.
Cook, A. A., J. C. Walker & R. H. Larson, 1952. Studies on the disease cycle of black rot of crucifers. Phytopathology 42: 162–167.
Doyle, J. & J. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.
Griffiths, P. D. & J. L. Nickels, 2001. Association of molecular poly-morphism with black rot resistance derived from Ethiopian mus-tard. Cruciferae Newsl 23: 57–58.
Guo, H., M. H. Dickson & J. E. Hunter, 1991. Brassica napus sources of resistance to black rot in crucifers and inheritance of resistance. HortScience 26: 1545–1547.
Hansen, L. N. & E. D. Earle, 1995. Transfer of resistance to Xan-thomonas campestris pv. campestris into Brassica oleracea L. by protoplast fusion. Theor Appl Genet 91: 1293–1300.
Hunter, J. E., M. H. Dickson & J. W. Ludwig, 1987. Sources of re-sistance to black rot of cabbage expressed in seedlings and adult plants. Plant Dis 71: 263–266.
Meng, J. & M. Lu, 1993. Genotype effects of Brassica napus on its reproductive behavior after pollination with B. juncea. Theor Appl Genet 87: 238–242.
Momotaz, A., M. Kato & F. Kakihara, 1998. Production of inter-generic hybrids between Brassica and Sinapis species by means of embryo rescue techniques. Euphytica 103: 123–130.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473–497.
Shelton, A. M. & J. E. Hunter, 1985. Evaluation of the potential of the flea bettle Phyllotreta cruciferae to transmit Xanthomonas campestris pv. campestris, casual agent of black rot of crucifers. Can J Plant Pathol 7: 308–310.
Song, K. M., T. C. Osborn & P. H. Williams, 1988. Brassica taxon-omy based on nuclear restriction fragment length polymorphisms (RFLPs). Theor Appl Genet 75: 784–794.
Taylor, J. D., J. Conway, S. J. Roberts, D. Astley & J. G. Vicente, 2002. Sources and origin of resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology 92: 105–111.
Tongu¸ c, M., E. D. Earle & P. D. Griffiths, 2003. Segregation distortion of Brassica carinata derived black rot resistance in B. oleracea. Euphytica 134: 269–276.
Vicente, J. G., J. Conway, S. J. Roberts & J. D. Taylor, 2001. Iden-tification and origin of Xanthomonas campestris pv. campestris races and related pathovars. Phytopathology 91: 492–499.
Warwick, S. I. & L. D. Black, 1991. Molecular systematics of Bras-sica and allied genera (subtribe Brassicenae, Brassicaceae)-chloroplast genome and cytodome congruence. Theor Appl Genet 82: 81–92.
Westman, A. L., S. Kresovich & M. H. Dickson, 1999. Regional vari-ation in Brassica nigra and other weedy crucifers for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Euphytica 106: 253–259.
Williams, P. H., 1980. Black rot: a continuing threat to world cru-cifers. Plant Dis 64: 736–742.
Zhou, Z., N. F. Weeden & M. H. Dickson, 1997. The expression of a resistant gene to black rot in progeny of the protoplast fusion broccoli (B. oleracea). Cruciferae Newsl 19: 109–110.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tonguç, M., Griffiths, P.D. Development of black rot resistant interspecific hybrids between Brassica oleracea L. cultivars and Brassica accession A 19182, using embryo rescue. Euphytica 136, 313–318 (2004). https://doi.org/10.1023/B:EUPH.0000032733.47031.5f
Issue Date:
DOI: https://doi.org/10.1023/B:EUPH.0000032733.47031.5f