Abstract
The type of dormancy and conditions necessary for germination of Agaricus bisporus basidiospores were studied. Basidiospores failed to germinate on starvation agar and required the presence of carbon and nitrogen sources (asparagine and/or glucose) in the medium. Upon 3-week storage, basidiospores germinated after 4–5 days. Heat shock (20 min at 45°C) and decreased temperature facilitated activation of germination. Heterocyclic compounds stimulating germination of endogenously dormant spores, such as furfural, failed to activate germination. The data obtained suggested an endogenous dormancy of A. bisporus basidiospores differing from zygospores of Mucorales. Basidiospores contained 17–19% lipids with a composition of fatty acids differing from those of the pileus and stipe of the fruiting body. The soluble carbohydrates of the cytosol amounted to 12% dry spore weight and consisted of mannitol (74%) and trehalose (26%). Unlike basidiospores stored at 2°C, basidiospores stored for 5 months at 20°C lost their ability to germinate, which correlated with a decrease in the content of trehalose.
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REFERENCES
Kursanov, L.N., Mikologiya (Mycology), Moscow: Izd. Narkomprosa RSFSR, 1940.
Ushatinskaya, P.S., Skrytaya zhizn' i anabioz (Hidden Life and Anabiosys), Moscow: Nauka, 1990.
Feofilova, E.P., Prikl. Biokhim. Mikrobiol., 2003, vol. 39, no. 1, pp. 5-24.
Garton, G.H., Goodwin, T.W., and Lijinsky, W., Biochem. J., 1951, vol. 48, no. 2, pp. 154-163.
Folch, J., Lees, M., and Sloane-Stanlet, G.H.S., J. Biol. Chem., 1957, vol. 226, no. 1, pp. 497-529.
Kates, M., Techniques of Lipidology, Amsterdam: Elsevier, 1972.
Somogyi, H., J. Biol. Chem., 1945, vol. 160, p. 69.
Methods in Carbohydrate Chemistry, vol. VI: General carbohydrate methods, Whistler, R. and Bemiller, J., Eds., New York: Academic: 1972. Translated under the title Metody issledovaniya uglevodov, Khorlina, F.Ya., Ed., Moscow: Mir, 1975, pp. 9-13.
Ashmarin, I.P. and Vorob'ev, A.A., Statisticheskie metody v mikrobiologicheskikh issledovaniyakh (Statistical Methods in Microbiological Studies), Leningrad: Meditsyna, 1962.
Klyushnikova, E.S., Uchenye zapiski MGU. Botanika (Research Letters of Moscow State Univ.), Moscow: Mosk. Gos. Univ., 1940, vol. 36, pp. 136-171.
Morozova, E.V., Baranova, M.V., Kozlov, V.P., Tereshina, V.M., Memorskaya, A.S., and Feofilova, E.P., Mikrobiologiya, 2000, vol. 70, no. 5, pp. 611-619.
Mills, Y.L. and Eilers, F.J., J. Gen. Microbiol., 1973, vol. 77, pp. 393-401.
Mikhailova, M.V., Feofilova, E.P., Shumskaya, G.G., and Shevtsov, V., Prikl. Biokhim. Mikrobiol., 1993, vol. 29, no. 2, pp. 315-320.
Sussman, A.S. and Halvorson, U.O., Spores. Their Dormancy and Germination, New York: Harper and Row, 1966.
Stayer, E.J., Am. J. Bot., 1930, vol. 18, no. 10, pp. 23-28.
Garibova, L.V., Biol. Nauki, 1964, no. 2, pp. 137-141.
Van Laere, A., FEMS Microbiology, 1986, vol. 38, pp. 251-256.
Safrai, A.I., Biological Characteristics of Some Species of the Genus Agaricus Fr. em. Karet, Cand. Sci. (Biol.) Dissertation, Moscow: Moscow State Univ., 1977.
Sussman, A.S., Spores, Their Dormancy and Germination, London: Harper and Row, 1966, pp. 102-138.
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Feofilova, E.P., Tereshina, V.M., Garibova, L.V. et al. Germination of Basidiospores of Agaricus bisporus . Applied Biochemistry and Microbiology 40, 186–191 (2004). https://doi.org/10.1023/B:ABIM.0000018924.36868.fd
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DOI: https://doi.org/10.1023/B:ABIM.0000018924.36868.fd