Skip to main content
Log in

Relationship between Calcium and Pyruvate Kinase

  • Published:
Biologia Plantarum

Abstract

Tobacco plants (Nicotiana tabacum L. cv. Sevilla) were grown under controlled conditions. The leaf content of Ca2+, Mg2+ and K+, and the activity of the pyruvate kinase were analyzed. The increased application of Ca2+ diminished the content of K+ and Mg2+ in leaves, and decreased the activity of pyruvate kinase. Taking into account these results, we suggest the pyruvate kinase activity as an bioindicator of the contents of the Ca2+, Mg2+ and K+.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bar-Akiva, A., Sagiv, J., Hasday, D.: Effect of mineral nutrient deficiencies on pyruvic kinase activity of citrus leaves.-In: Procedings of 4th International Colloquion on the Control of Plant Nutrition. Volume 1. Pp. 109-118. Rijksuniversiteit, Gent 1976.

    Google Scholar 

  • Baysdorfer, C., Bassham, J.A.: Spinach pyruvate kinase isoforms. Partial purification and regulatory properties.-Plant Physiol. 74: 374-379, 1984.

    Google Scholar 

  • Besford, R.T.: Phosphorus nutrition and acid phosphatase activity in leaves of seven plant species.-J. Sci. Food Agr. 30: 282-285, 1979.

    Google Scholar 

  • Bharti, N., Singh, R.P., Sinha, S.K.: Effect of calcium chloride on heavy metal induced alteration in growth and nitrate assimilation of Sesamum indicum seedlings.-Phytochemistry 41: 105-109, 1996.

    Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dyebinding.-Anal. Biochem. 72: 248-254, 1976.

    Google Scholar 

  • Fenn, L.B., Taylor, R.M., Burks, C.M.: Calcium stimulation of ammonium absorption and growth by sugar beet.-Agron. J. 86: 916-920, 1994.

    Google Scholar 

  • Geigenberger, P., Stitt, M.: Regulation of carbon partitioning between sucrose and nitrogen assimilation in cotyledons of germinating Ricinus communis L. seedlings.-Planta 185: 563-568, 1991.

    Google Scholar 

  • Hocking, P.J., Pate, J.S.: Mobilization of minerals to developing seeds of legumes.-Ann. Bot. 41: 1259-1278, 1977.

    Google Scholar 

  • Kafkafi, U., Siddiqi, M.Y., Ritchie, R.J., Glass, A.D.M., Ruth, T.J.: Reduction of nitrate (13NO3) influx and nitrogen (13N) translocation by tomato and melon varieties after short exposure to calcium and potassium chloride salts.-J. Plant Nutr. 15: 959-975, 1992.

    Google Scholar 

  • Lachica, M., Aguilar, A., Yañez, J.: [Leaf analysis. Methods used in the Experimental Station in Zaidin.]-Anal. Edafol. Agrobiol. 32: 1033-1047, 1973. [In Span.]

    Google Scholar 

  • Lavon, R., Goldschmidt, E.E.: Enzymatic methods for detection of mineral element deficiencies in citrus leaves: a mini-review.-J. Plant Nutr. 22: 139-150, 1999.

    Google Scholar 

  • Lin, M., Turpin, D.H., Plaxton, W.C.: Pyruvate kinase isoenzymes from the green alga Selenastrum minutum. II. Kinetic and regulatory properties.-Arch. Biochem. Biophys. 269: 228-238, 1989.

    Google Scholar 

  • Marschner, H., Kirkby, E.A., Cakmak, I.: Effect of mineral nutritional status on shoot-root partitioning of photoassimilates and cycling of mineral nutrients.-J. exp. Bot. 47: 1255-1263, 1996.

    Google Scholar 

  • Podestá, F.E., Plaxton, W.C.: Kinetic and regulatory properties of cytosolic pyruvate kinase from germinating castor beans.-Biochem. J. 279: 495-501, 1991.

    Google Scholar 

  • Podestá, F.E., Plaxton, W.C.: Plant cytosolic pyruvate kinase: A kinetic study.-Biochim. biophys. Acta 1160: 213-220, 1992.

    Google Scholar 

  • Pulgar, G., Belakbir, A., Villora, G., Moreno, D., Romero, L.: Pyruvate kinase activity as bioindicator of cations in grafted watermelon plants.-Commun. Soil Sci. Plant Anal. 27: 1027-1046, 1996.

    Google Scholar 

  • Romero, L., Belakbir, A., Ragala, L., Ruiz, J.M.: Response of plant yield and leaf pigments to saline conditions: effectiveness of different rootstocks in melon plants (Cucumis melo L.).-Soil Sci. Plant Nutr. 43: 111-116, 1997.

    Google Scholar 

  • Ruiz, J.M., Moreno, D.A., Romero, L.: Pyruvate kinase activity as an indicator of the level of cations (K+, Mg2+ and Ca2+) in leaves and fruits of the cucumber: the role of potassium fertilization.-J. agr. Food Chem. 47: 845-849, 1999a.

    Google Scholar 

  • Ruiz, J.M., Rivero, R.M., Garcia, P.C., Baghour, M., Romero, L.: Nitrogen metabolism in leaves and roots of tobacco plants (Nicotiana tabacum L.): Role of calcium as a possible regulatory factor.-Plant Sci. 141: 107-115, 1999b.

    Google Scholar 

  • Schuller, K.A., Plaxton, W.C., Turpin, D.H.: Regulation of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum: properties associated with the replenishment of tricarboxylic acid cycle intermediates during ammonium assimilation.-Plant Physiol. 93: 1303-1311, 1990.

    Google Scholar 

  • Sung, F.J.M., Lo, W.S.: Growth responses of rice in ammonium based nutrient solution with variable calcium supply.-Plant Soil 125: 239-244, 1990.

    Google Scholar 

  • Turpin, D.H., Botha, F.C., Smith, R.G., Feil, R., Horsey, A.K., Vanlerberghe, G.C.: Regulation of carbon partitioning to respiration during dark ammonium assimilation in the green alga Selenastrum minutum.-Plant Physiol. 93: 166-175, 1990.

    Google Scholar 

  • Vanlerberghe, G.C., Schuller, K.A., Smith, R.G., Feil, R., Plaxton, W.C., Turpin, D.H.: Relationship between NH4 + assimilation rate and in vivo phosphoenolpyruvate carboxylase activity. Regulation of anapleurotic carbon flow in the green alga Selenastrum minutum.-Plant Physiol. 94: 284-290, 1990.

    Google Scholar 

  • Van Quy, L., Foyer, C., Champigny, M.L.: Effect of light and NO3- on wheat leaf phosphoenolpyruvate carboxylase activity. Plant Physiol. 97: 1476-1482, 1991.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ruiz, J., López-Cantarero, I. & Romero, L. Relationship between Calcium and Pyruvate Kinase. Biologia Plantarum 43, 359–362 (2000). https://doi.org/10.1023/A:1026757320303

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026757320303

Navigation