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Mapping candidate genes in Eucalyptus with emphasis on lignification genes

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Abstract

We used the single-strand conformation polymorphism (SSCP) technique to map eight genes on Eucalyptus urophylla and Eucalyptus grandis linkage maps. These included four genes involved in the common phenylpropanoid pathway (caffeic acid 3-0-methyltransferase, caffeoyl CoA 3-O-methyltransferase, 4-coumarate CoA ligase and phenylalanine ammonia-lyase), two genes involved in the `lignin specific' pathway (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase), and two symbiosis regulated genes (EgHypar and EgTubA1). A novel source of variation which affects the SSCP pattern, i.e. the presence or absence of electrophoresis buffer upon loading the samples into the polyacrylamide gel, was found. The placement of these genes on the Eucalyptus maps was carried out using an interspecific hybrid mapping population. This will further facilitate the identification or exclusion of `positional' candidate genes for characterizing quantitative trait loci (QTL) for wood quality and vegetative propagation related traits.

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Authors and Affiliations

  1. Programme Arbres et Plantations, CIRAD-Forêt, BP 5035, 34032, Montpellier, France

    Jean-Marc Gion, Philippe Rech & Daniel Verhaegen

  2. Pôle de Biotechnologie Végétale, UMR CNRS/Université Paul Sabatier no. 5546, BP 17, Auzeville, 31326 , Castanet Tolosan, France

    Philippe Rech & Jacqueline Grima-Pettenati

  3. Laboratoire de Génétique et Amélioration des Arbres Forestiers, INRA, BP 45, 33610, Cestas, France

    Christophe Plomion

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  1. Jean-Marc Gion
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Gion, JM., Rech, P., Grima-Pettenati, J. et al. Mapping candidate genes in Eucalyptus with emphasis on lignification genes. Molecular Breeding 6, 441–449 (2000). https://doi.org/10.1023/A:1026552515218

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