Skip to main content
SpringerLink
Log in

Proliferation and Insulin Secretion Function of Mouse Insulinoma Cells Encapsulated in Alginate/Sol-Gel Synthesized Aminopropyl-Silicate/Alginate Microcapsule

  • Published:
Journal of Sol-Gel Science and Technology Aims and scope Submit manuscript

Abstract

Alginate/aminopropyl-silicate/alginate microcapsules, ca. 15 μm in membrane thickness and ca. 500 μm in diameter, were prepared via sol-gel process. The aminopropyl-silicate membrane was derived from two silicone alkoxide precursors, tetramethoxysilane and 3-aminopropyl-trimethoxysilane on Ca-alginate micro gel beads. Pancreatic β-cell line (MIN6) cells were encapsulated in the microcapsule. The encapsulated MIN6 cells proliferated and formed spheroidal tissues in vitro. The diameter of the MIN6 spheroids increased to approximately 250 μm with an increase in the incubation period until the day 35. Storeptozotocin-induced diabetic mice became normoglycemia after implantation of the MIN6-enclosing microcapsules. The normoglycemic state remained until the retrieval of the implanted microcapsules for 1 month. These results indicate that the potential use of the alginate/aminopropyl-silicate/alginate microcapsule as a vehicle for a genetically engineered cell-enclosing therapeutic material delivery system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C.J. Brinker, K.D. Keefer, D.W. Schaefer, and C.S. Ashley, J. Non-Cryst. Sol. 48, 47 (1982).

    Google Scholar 

  2. C. Roux, J. Livage, K. Farhati, and L. Monjour, J. Sol-Gel Sci. Techn. 8, 663 (1997).

    Google Scholar 

  3. D.M. Liu and I.W. Chen, Acta. Mater. 47, 4535 (1999).

    Google Scholar 

  4. S. Fennouh, S. Guyon, J. Livage, and C. Roux, J. Sol-Gel Sci. Techn. 19, 647 (2000).

    Google Scholar 

  5. U. Georgi, H. Graebner, G. Roewer, and G. Wolf, J. Sol-Gel Sci. Techn. 13, 295 (1998).

    Google Scholar 

  6. I. Gill and A. Ballesteros, Trends Biotechnol. 18, 282 (2000).

    Google Scholar 

  7. E.J.A. Pope, J. Sol-Gel Sci. Techn. 8, 635 (1997).

    Google Scholar 

  8. G. Carturan, G. Dellagiacoma, M. Rossi, R. Dal Monte, and M. Muraca, SPIE Sol-Gel Opt. IV 336, 31 (1997).

    Google Scholar 

  9. S. Sakai, T. Ono, H. Ijima, and K. Kawakami, Biomaterials 22, 2827 (2001).

    Google Scholar 

  10. F. Lim and A.M. Sun, Science 210, 908 (1980).

    Google Scholar 

  11. S.M. Chia, K.W. Leong, J. Li, X. Xu, K. Zeng, P.N. Er, S. Gao, and H. Yu, Tissue Eng. 6, 481 (2000).

    Google Scholar 

  12. K. Aoki, K. Hakamada, Y. Umehara, K. Seino, Y. Itabashi, and M. Sasaki, Transplant. Proc. 32, 1118 (2000).

    Google Scholar 

  13. Y.L. Xue, J.M. Gao, Z.F. Xi, Z.F. Wang, X.J. Li, X. Cui, Y. Luo, C.H. Li, L.N. Wang, D. Zhou, R. Sun, and A.M. Sun, Artif. Organs 25, 131 (2001).

    Google Scholar 

  14. A. Gaumann, M. Laudes, B. Jacob, R. Pommersheim, C. Laue, W. Vogt, and J. Schrezenmeir, Exp. Toxicol. Pathol. 53, 35 (2001).

    Google Scholar 

  15. T.M.S. Chang, and S. Prakash, Mol. Med. Today 4, 221 (1998).

    Google Scholar 

  16. P.L. Chang, J.M. Van Raamsdonk, G. Hortelano, S.C. Barsoum, N.C. MacDonald, and T.L. Stockley, Trends Biotechnol. 17, 78 (1999).

    Google Scholar 

  17. A. Al-Hendy, G. Hortelano, G.S. Tannenbaum, and P.L. Chang, Hum. Gene Ther. 7, 61 (1996).

    Google Scholar 

  18. G. Hortelano, N. Xu, A. Vandenberg, J. Solera, P.L. Chang, and F.A. Ofosu, Hum. Gene Ther. 10, 1281 (1999).

    Google Scholar 

  19. B. Ríhová, Adv. Drug Deliver. Rev. 42, 65 (2000).

    Google Scholar 

  20. H. Uludag, P. De Vos, and P.A. Tresco, Adv. Drug. Deliv. Rev. 42, 29 (2000).

    Google Scholar 

  21. A.M. Sun, G.M. O'shea, and M.F.A. Goosen, Appl. Biochem. Biotechnol. 10, 87 (1984).

    Google Scholar 

  22. I. Lacik, M. Brissova, A.C. Powers, A.V. Anilkumar, and T.J. Wang, J. Biomed. Mater. Res. 39, 52 (1998).

    Google Scholar 

  23. M. Darrabie, B.K. Freeman, W.F. Kendall, H.A. Hobbs, and E.C. Opara, J. Biomed. Mater. Res. 54, 396 (2000).

    Google Scholar 

  24. Y.J. Wang, Mater. Sci. Eng. C 13, 59 (2000).

    Google Scholar 

  25. J. Miyazaki, K. Araki, E. Yamato, H. Ikegami, T. Asano, Y. Shibasaki, Y. Oka, and K. Yamamura, Endocrinology 127, 126 (1990).

    Google Scholar 

  26. S. Sakai, T. Ono, H. Ijima, and K. Kawakami, J. Membrane. Sci. 202, 73 (2002).

    Google Scholar 

  27. N. Kinoshita, Y. Echigo, S. Shinohara, Y. Gu, J. Miyazaki, K. Inoue, and M. Imamura, Cell. Transplant. 10, 473 (2001).

    Google Scholar 

  28. G. Arcangeli, V. Cupelli, and G. Giuliano, Sci. Total Environ. 270, 135 (2001).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima & Koei Kawakami

Authors
  1. Shinji Sakai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tsutomu Ono
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroyuki Ijima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Koei Kawakami
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sakai, S., Ono, T., Ijima, H. et al. Proliferation and Insulin Secretion Function of Mouse Insulinoma Cells Encapsulated in Alginate/Sol-Gel Synthesized Aminopropyl-Silicate/Alginate Microcapsule. Journal of Sol-Gel Science and Technology 28, 267–272 (2003). https://doi.org/10.1023/A:1026045503586

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026045503586

Search

Navigation