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Arrowroot (Marantha arundinacea) starch as a new low-cost substrate for alkaline protease production

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Abstract

The feasibility of arrowroot (Marantha arundinacea) starch for alkaline protease production using an alkalophilic Bacillus lentus isolate was evaluated in batch fermentations in shake flasks and in a bioreactor under a range of conditions. A new arrowroot starch-casein medium (pH 10.2) was formulated having a composition (%, w/v): arrowroot starch 1, casein 1, sodium succinate 0.25, NH4Cl 0.05, NaCl 0.05, KH2PO4 0.04, K2HPO4 0.03, MgCl2 0.01, yeast extract 0.01 and Na2CO3 1.05. The isolate produced a maximum protease yield (6754.7 U ml−1) in this medium when grown for 72 h at 250 rev/min and 37 °C. Scaling-up studies in a bioreactor showed a 5-fold increase in alkaline protease yields (31899 U ml−1) at a lower production time of 45 h, aeration of 1 v/v/m and agitation of 400 rev/min at 37 °C.

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References

  • Banerjee, U.C., Sani, R.K., Azmi, W. & Soni, R. 1999 Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive. Process Biochemistry 35, 213–219.

    Google Scholar 

  • Bartholomew, W.H. 1960 Scale-up of submerged fermentations. Advances in Applied Microbiology 2, 289–300.

    Google Scholar 

  • Chandrasekaran, S. & Dhar, S.C. 1983 A low-cost method for the production of extracellular alkaline proteinase using tapioca starch. Journal of Fermentation Technology 61, 511–514.

    Google Scholar 

  • Fujiwara, N. & Yamamoto, K. 1987 Production of alkaline protease in a low-cost medium by alkalophilic Bacillus sp. and properties of the enzyme. Journal of Fermentation Technology 65, 345–348.

    Google Scholar 

  • Gessesse, A. 1997 The use of nug meal as low-cost substrate for the production of alkaline protease by the alkaliphilic Bacillus sp. AR-009 and some properties of the enzyme. Bioresource Technology 62, 59–61.

    Google Scholar 

  • Hodgson, J. 1994 The changing bulk biocatalyst market. Biotechnology 12, 789–790.

    Google Scholar 

  • Jasvir, S., Gill, N., Devasahyam, G. & Sahoo, D.K. 1999 Studies on alkaline protease produced by Bacillus sp. NG312. Applied Biochemistry and Biotechnology 76, 57–63.

    Google Scholar 

  • Johnvesly, B., Manjunath, B.R. & Naik, G.R. 2002 Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophilic Bacillus sp. JB-99. Bioresource Technology 82, 61–64.

    Google Scholar 

  • Joo, H.S., Kumar, C.G., Park, G.C., Kim, K.T., Paik, S.R. & Chang, C.S. 2002 Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii. Process Biochemistry 38, 155–159.

    Google Scholar 

  • Krulwich, T.A. 1996 Bioenergetics of alkalophilic bacteria. Journal of Membrane Biology 89, 113–125.

    Google Scholar 

  • Krulwich, T.A. & Guffanti, A.A. 1989 The Na+ cycle of extreme alkalophiles: a secondary Na+/H+ antiporter and Na+/solute symporters. Journal of Bioenergetics and Biomembranes 21, 663–678.

    Google Scholar 

  • Kumar, C.G. 2002 Purification and characterization of a thermostable alkaline protease from alkalophilic Bacillus pumilus. Letters in Applied Microbiology 34, 13–17.

    Google Scholar 

  • Kumar, C.G., Malik, R.K. & Tiwari, M.P. 1998 Novel enzyme-based detergents: an Indian perspective. Current Science 75, 1312–1318.

    Google Scholar 

  • Kumar, C.G., Malik, R.K., Tiwari, M.P. & Jany, K.D. 1999a Optimal production of Bacillus alkaline protease using a cheese whey medium. Microbiologie des Alimentes et Nutrition 17, 39–48.

    Google Scholar 

  • Kumar, C.G. & Takagi, H. 1999 Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnology Advances 17, 561–594.

    Google Scholar 

  • Kumar, C.G., Tiwari, M.P. & Jany, K.D. 1999b Novel alkaline serine proteases from alkalophilic Bacillus sp.: purification and characterization. Process Biochemistry 34, 441–449.

    Google Scholar 

  • Labbe, R., Somers, E. & Duncan C. 1976 Influence of starch source on sporulation and enterotoxin production by Clostridium perfringens type A. Applied and Environmental Microbiology 31, 455–457.

    Google Scholar 

  • Lajvardi, A., Mazarin, G.I., Gillespie, M.B., Satchithanandam, S. & Calvert, R.J. 1993 Starches of varied digestibilities differentially modify intestinal function in rats. Journal of Nutrition 123, 2059–2066.

    Google Scholar 

  • Mabrouk, S.S., Hashem, A.M., El-Shayeb, N.M.A., Ismail, A.M.S. & Abdel-Fattah, A.F. 1999 Optimization of alkaline protease productivity by Bacillus licheniformis ATCC 21415. Bioresource Technology 69, 155–159.

    Google Scholar 

  • Malathi, S. & Chakraborty, R. 1991 Production of alkaline protease by a new Aspergillus flavus isolate under solid-substrate fermentation conditions for use as a depilation agent. Applied and Environmental Microbiology 57, 712–716.

    Google Scholar 

  • Paavilainen, S., Makela, M. & Korpela, T. 1995 Proton and carbon inventory during the growth of an alkalophilic Bacillus indicates that protons are independent from acid anions. Journal of Fermentation and Bioengineering 80, 429–433.

    Google Scholar 

  • Peddie, C.J., Cook, G.M. & Morgan, H.W. 1999 Sodium-dependent glutamate uptake by an alkaliphilic, thermophilic Bacillus strain TA2.A1. Journal of Bacteriology 181, 3172–3177.

    Google Scholar 

  • Priest, F.G. 1977 Extracellular enzyme synthesis in the genus Bacillus. Bacteriology Reviews 41, 711–753.

    Google Scholar 

  • Prowe, S.G., van de Vossenberg, J.L.C.M., Driessen, A.J.M., Antranikian, G. & Konings, W.N. 1996 Sodium-coupled energy transduction in the newly isolated thermoalkaliphilic strain LBS3. Journal of Bacteriology 178, 4099–4104.

    Google Scholar 

  • Raja, M.K.C. & Sindhu, P. 2000 Properties of steam-treated arrowroot (Maranta arundinacea) starch. Starch/Stärke 52, 471–476.

    Google Scholar 

  • Sawada, J., Yasui, H., Amamoto, T., Yamada, M., Okazaki, T. & Tanaka, I. 1974 Isolation and some properties of anti-proteolytic polypeptides from potato. Agricultural and Biological Chemistry 38, 2559–2561.

    Google Scholar 

  • Tobisch, S., Zühlke, D., Bernhardt, J., Stülke, J. & Hecker, M. 1999 Role of CcpAin regulation of the central pathways of carbon catabolism in Bacillus subtilis. Journal of Bacteriology 181, 6996–7004.

    Google Scholar 

  • Tsujii, K. 2002 Donnan equilibria in microbial cell walls: a pH-homeostatic mechanism, in alkaliphiles. Colloids and Surfaces B: Biointerfaces 24, 247–251.

    Google Scholar 

  • van Putten, A.P.B., Spitzenberger, F., Kretzmer, G., Hitzmann, B., Dors, M., Simutis, R. & Schugerl, K. 1996 Improvement of the production of subtilisin Carlsberg alkaline protease by Bacillus licheniformis by on-line process monitoring and control in a stirred tank reactor. Journal of Biotechnology 49, 83–93.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, College of Medicine, Inha University, B-501, Jung Suk Building, 7-241 Shinheung-Dong 3 Ka, Jung-ku, Incheon, 400-103, South Korea

    C. Ganesh Kumar

  2. Department of Biochemistry, Bose Institute, P-1/12 CIT Scheme VII M, Calcutta, 700054, India; Tel.:

    C. Ganesh Kumar & Pradeep Parrack

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  1. C. Ganesh Kumar
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  2. Pradeep Parrack
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Kumar, C.G., Parrack, P. Arrowroot (Marantha arundinacea) starch as a new low-cost substrate for alkaline protease production. World Journal of Microbiology and Biotechnology 19, 757–762 (2003). https://doi.org/10.1023/A:1025156105148

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