Abstract
Apoptotic cell death in suspension cultures of Taxus cuspidata induced by exogenous salicylic acid and/or H2O2 was investigated. H2O2 (0.012% v/v) alone changed the permeability of cell membrane while salicylic acid (0.375 mM) not only altered the permeability but also caused nuclei condensation and a small amount of nuclei fragments. The combined use of salicylic acid (0.375 mM) and H2O2 (0.012% v/v) changed the cell membrane permeability more significantly and nuclei fragments occurred in ca. 30% of the cells at 48 h. DNA ladders of 180 bp and oligopolymers, characteristics of the apoptotic cleavage of nuclei DNA, were observed by agar electrophoresis. These results show that exogenous salicylic acid and H2O2 could synergistically induce the apoptotic cell death of suspension cultures of Taxus cuspidata.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Durner J, Klessig DF (1995) Inhibition of ascorbate peroxidase by salicylic acid and 2,6-dichloroisonicotinic acid, two inducers of plant defense responses. Proc. Natl. Acad. Sci. USA 92: 11312–11316.
Friedrich L, Vernooij B, Gaffney T, Morse A, Ryals J (1995) Characterization of tobacco plants expressing a bacterial salicylate hydroxylase gene. Plant Mol. Biol. 29: 959–968.
Ganesan V, Thomas G (2001) Salicylic acid response in rice: in-fluence of salicylic acid on H2O2 accumulation and oxidative stress. Plant Sci. 160: 1095–1106.
Jabs T (1999) Reactive oxygen intermediates as mediators of programmed cell death in plants and animals. Biochem. Pharm. 57: 231–245.
Jabs T, Dietrich RA, Dangl JL (1996) Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide. Science 273: 1853–1856.
Jones A (2000) Does the plant mitochondrion integrate cellular stress and regulate programmed cell death? Trends Plant Sci. 5: 225–230.
Kauss H, Jeblick W (1995) Pretreatment of parsley suspension cultures with salicylic acid enhances spontaneous and elicited production of H2O2. Plant Physiol. 108: 1171–1178.
Lamb C, Dixon RA (1997) The oxidative burst in plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 251–275.
Levine A, Tenhaken R, Dixon RA, Lamb CJ (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79: 583–593.
Ludwig A, Tenhaken R (2000) Defence gene expression in soybean is linked to the status of the cell death program. Plant Mol. Biol. 44: 209–218.
Mur L, Naylor G, Warner SAJ, Sugars JM, White RF, Draper J (1996) Salicylic acid potentiates defense gene expression in tissue exhibiting acquired resistance to pathogen attack. Plant J. 9: 559–571.
Sticher L, Mauch-Mani B, Métraux JP (1997) Systemic acquired resistance. Annu. Rev. Plant. Pathol. 35: 235–270.
Summermatter K, Sticher L, Métraux JP (1995) Systemic responses in Arabidopsis thaliana infected and challenged with Pseudomonas syringae psyringae. Plant Physiol. 108: 1379–1385.
Yuan YJ, Ge ZQ, Wang YD, Ma ZY, Hu ZD (2001) Ce4+ induces apoptosis in suspension culture of Taxus cuspidata cells. J. Chin. Rare Earth Soc. 19: 357–361.
Yuan YJ, Ma ZY, Wu JC, Zeng AP (2002) Taxol-induced apoptotic cell death in suspension cultures of Taxus cuspidata. Biotechnol. Lett. 24: 615–618.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Qiao, JJ., Yuan, YJ., Zhao, H. et al. Apoptotic cell death in suspension cultures of Taxus cuspidata co-treated with salicylic acid and hydrogen peroxide. Biotechnology Letters 25, 387–390 (2003). https://doi.org/10.1023/A:1022494213446
Issue Date:
DOI: https://doi.org/10.1023/A:1022494213446