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Effective Protocol for in Vitro Shoot Production Through Nodal Explants of Simmondsia Chinensis

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Biologia Plantarum

Abstract

Nodal explants excised from 18 to 20-year-old female plants of Simmondsia chinensis if cultured on Murashige and Skoog's medium supplemented with 20 μM N6-benzyladenine (BA) differentiated an average of 2.7 ± 0.4 shoots in 11.5% explants. The percentage of nodal explants inducing multiple shoots enhanced significantly if in vitro raised shoots were used as source of explants. Nearly 100% cultures differentiated an average of 4.7 ± 2.0 shoots per explant on the same medium. Nearly 85% of the shoots induced roots when a pulse treatment of 50 μM indole-3-butyric acid (IBA) was given prior to their transfer to semi-solid MS medium containing 10 μM IBA + 0.5% activated charcoal + 1 μM BA. Plantlets were gradually hardened in Soilrite and acclimatized to soil.

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    V. Agrawal, S. Prakash & S.C. Gupta

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  1. V. Agrawal
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  2. S. Prakash
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  3. S.C. Gupta
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Agrawal, V., Prakash, S. & Gupta, S. Effective Protocol for in Vitro Shoot Production Through Nodal Explants of Simmondsia Chinensis . Biologia Plantarum 45, 449–453 (2002). https://doi.org/10.1023/A:1016238205522

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