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Optimization of dilution rate for the production of value added product and simultaneous reduction of organic load from pineapple cannery waste

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Abstract

Candida utiilis NRRL Y-900 was grown on pineapple cannery waste as the sole carbon and energy source in a chemostat at dilution rates ranging between 0.05 and 0.65 h−1 to determine the growth kinetics. The cell yield coefficient varied with dilution rate and a maximum value of 0.662 ± 0.002 gx/gcarb was obtained at a dilution rate of 0.4 h−1. At steady state, the concentrations of carbohydrate, reducing sugar, and chemical oxygen demand (COD) appeared to follow Monod kinetics. At maximum specific growth rate (μmax) 0.65 h−1, the saturation constants for carbohydrate, reducing sugar and COD were 0.51 ± 0.02 gcarb/1, 0.046 ± 0.003 grs/1, and 1.036 ± 0.001 gCOD/1, respectively. Maximum biomass productivity (Q x max) 2.8 ± 0.03 gx/1 h was obtained at a dilution rate of 0.5 h−1. At this dilution rate, only 71.0 ± 0.41% COD was removed whereas at a dilution rate of 0.1 h−1, 98.2 ± 0.35% reduction in COD was achieved. At a dilution rate of 0.4 h−1, the optimal yeast productivity and reduction in COD were 2.7 ± 0.13 gp/1 h, and 84.2 ± 0.42%, respectively.

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Authors and Affiliations

  1. Biochemistry Division, Regional Research Laboratory, Jorhat, 785 006, Assam, India

    J.N. Nigam

  2. Computer Section, Regional Research Laboratory, Jorhat, 785 006, Assam, India

    M.C. Kakati

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  1. J.N. Nigam
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  2. M.C. Kakati
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Nigam, J., Kakati, M. Optimization of dilution rate for the production of value added product and simultaneous reduction of organic load from pineapple cannery waste. World Journal of Microbiology and Biotechnology 18, 303–308 (2002). https://doi.org/10.1023/A:1015201215368

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