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Induction of mRNAs in response to acclimation of trout cells to different osmolalities

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Abstract

The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.

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Authors and Affiliations

  1. National Research Institute of Fisheries Science, Yokohama, 236-0004, Japan

    T. Sakamoto, N. Ojima & M. Yamashita

  2. Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-hiroshima, 739-8521, Japan

    T. Sakamoto, N. Ojima & M. Yamashita

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  1. T. Sakamoto
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  2. N. Ojima
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Sakamoto, T., Ojima, N. & Yamashita, M. Induction of mRNAs in response to acclimation of trout cells to different osmolalities. Fish Physiology and Biochemistry 22, 255–262 (2000). https://doi.org/10.1023/A:1007843508043

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