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Identification of tomato Lhc promoter regions necessary for circadian expression

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Abstract

Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5′-upstream promoter regions are present at different positions relative to the transcription start site of Lhc b11, b12, Lhc a3 and Lhc a4. A short sequence of 47 nucleotides is necessary for conferring circadian Lhc mRNA oscillations. Sequence alignment of the specified promoter regions revealed a novel motif ‘CAANNNNATC’. This motif is conserved in 5′-upstream regions of clock controlled Lhc genes and overlaps with a sequence relevant in phytochrome mediated gene expression.

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Author notes

  1. Barbara Rudolph

    Present address: Institut für Pflanzenbau und Pflanzenzüchtung, Von-Siebold-Strasse 8, 37077, Göttingen, Germany

Authors and Affiliations

  1. Universität Rostock, Biochemie, Fachbereich Biologie, Gertrudenstrasse 11a, 18051, Rostock, Germany

    Birgit Piechulla & Nicole Merforth

Authors
  1. Birgit Piechulla
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  2. Nicole Merforth
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  3. Barbara Rudolph
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Piechulla, B., Merforth, N. & Rudolph, B. Identification of tomato Lhc promoter regions necessary for circadian expression. Plant Mol Biol 38, 655–662 (1998). https://doi.org/10.1023/A:1006094015513

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