Abstract
The effect of genistein and genistin on bone components in the femoral-metaphyseal tissues obtained from elderly female rats was investigated in vitro. The metaphyseal tissues were cultured for 24 h in a medium containing either vehicle, genistein (10-8-10-5 M) or genistin (10-7-10-5 M). The presence of genistein or genistin caused a significant increase in alkaline phosphatase activity, deoxyribonucleic acid (DNA) and calcium contents in the metaphyseal tissues. The effect of genistein was greater than that of genistin. The bone components increased by genistein (10-5 M) or genistin (10-5 M) were completely blocked by the presence of cycloheximide (10-6 M). The presence of zinc sulfate (10-5 M) caused a significant increase in the genistein (10-5 M)-elevated alkaline phosphatase activity, DNA and calcium contents. The enhancement with zinc was not seen by genistin (10-5 M). The stimulatory effect of zinc on the genistein-induced increase in bone components of the metaphyseal tissues was completely blocked by the presence of cycloheximide (10-6 M). The present results suggest that genistein and genistin have an anabolic effect on bone metabolism in the femoral-metaphyseal tissues of elderly rats, and that the genistein effect is enhanced by zinc, an essential trace element.
Similar content being viewed by others
References
Albright F, Smith PH, Richardson AM: Postmenopausal osteoporosis: its clinical features. J Am Med Assoc 116: 2465–2474, 1941
Riggs BL, Jowsey J, Kelly PJ, Jones JD, Maher FT: Effect of sex hormones on bone in primary osteoporosis. J Clin Invest 48:1065–1072, 1969
Liu Y, Bhalla K, Hill C, Priest DG: Evidence for involvement of tyrosine phosphorylation in taxol-induced apoptosis in a human ovarian tumor cell line. Biochem Pharmacol 48:1265–1272, 1994
Spinozzi F, Pagliacci MC, Migliorati G, Moraca R, Grignami F, Riccardi C, Nicoletti I: The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells. Leuk Res 18:431–439, 1994
Bergamschi G, Rosti V, Danova M, Ponchio L, Lucotti C, Cazzola M: Inhibitors of tyrosine phosphorylation induce apoptosis in human leukemic cell lines. Leukemia 7:2012–2018, 1993
Yamaguchi M, Oishi H, Suketa Y: Stimulatory effect of zinc on bone formation in tissue culture. Biochem Pharmacol 36:4007–4012, 1987
Yamaguchi M, Hashizume M: Effect of ?-alanyl-L-histidinato zinc on protein components in osteoblastic MC3T3-E1 cells: Increase in osteocalcin, insulin-like growth factor-I and transforming growth factor-β. Mol Cell Biochem 136:163–169, 1994
Kishi S, Yamaguchi M: Inhibitory effect of zinc compounds on osteoclast-like cell formation in mouse marrow cultures. Biochem Pharmacol 48:1225–1230, 1994
Yamaguchi M, Kishi S: Zinc compounds inhibit osteoclast-like cell formation at the earlier stage of rat marrow culture but not osteoclast function. Mol Cell Biochem 158:171–177, 1996
Yamaguchi M, Kishi S: Prolonged administration of ?-alanyl-Lhistidinato zinc prevents bone loss in ovariectomized rats. Japan J Pharmacol 63:203–207, 1993
Walter K, Schutt C: Acid and alkaline phosphatase in serum. In: HU Bergmeyer (ed). Methods of Enzymatic Analysis, Vols 1 and 2. Academic Press, New York, 1965, pp 856–860
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–273, 1951
Flanagan B, Nichols G Jr: Metabolic studies of bone in vitro. VI: Collagen biosynthesis by surviving bone fragment in vitro. J Biol Chem 237:3786–3789, 1962
Ceriotti G: Determination of nuclei acids in animal tissues. J Biol Chem 214:39–77, 1955
Yamaguchi M: ?-Alanyl-L-histidinato zinc and bone resorption. Gen Pharmac 26:1179–1183, 1995
Burch RE, Hahn HKJ, Sullivan JF: Newer aspects of the roles of zinc, manganese and copper in human nutrition. Clin Chim 21:501–520, 1975
Oner G, Bhaumick B, Bala EM: Effect of zinc deficiency on serum somatomedin levels and skeletal growth in young rats. Endocrinology 114:1860–1863, 1984
Yamaguchi M, Kishi S, Hashizume M: Effect of zinc-chelating dipeptides on osteoblastic MC3T3-E1 cells: Activation of aminoacyltRNA synthetase. Peptides 15:1367–1371, 1994
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamaguchi, M., Hua Gao, Y. Anabolic effect of genistein and genistin on bone metabolism in the femoral-metaphyseal tissues of elderly rats: The genistein effect is enhanced by zinc. Mol Cell Biochem 178, 377–382 (1998). https://doi.org/10.1023/A:1006809031836
Issue Date:
DOI: https://doi.org/10.1023/A:1006809031836