Abstract
A single dominant blast resistance gene conferring resistance to a Korean rice blast isolate was identified in rice variety `Suweon 365'. We report the chromosomal localization and molecular mapping of this blast resistance gene designated as Pi-18, which confers resistance to Korean isolate `KI-313' of the blast pathogen. To know whether there is a relationship among genes conditioning resistance to location-specific isolates of the blast pathogen and thereby to identify linked markers to resistance gene for isolate KI-313 collected in Korea, RFLP markers previously reported to be linked to major blast resistance genes in different rice germplasm and other markers mapped to nearby regions were surveyed for polymorphism between a resistant (`Suweon 365') and a susceptible (`Chucheongbyeo') parent. Linkage associations of the RFLP markers with the resistance gene were verified using an F2 and F3 segregating population of known blast reaction. RFLP analysis showed that Pi-18 was located near the end of chromosome 11, linked to a single copy clone RZ536 at a distance of 5.4 centiMorgans (cM) and that this gene was different from Pi-1(t). An allelism test revealed that this gene was also different from Pi-k. Currently, a combination of RAPD and microsatellite primers is being employed to find additional markers in this region. Tightly linked DNA markers will facilitate selection for resistant genotypes in breeding programs and provide the basis for map based cloning of this new blast resistance gene.
Similar content being viewed by others
References
Ahn, S.N., Y.K. Kim, S.S. Han, H.C. Choi, H.P. Moon & S.R. McCouch, 1996a. Molecular mapping of a gene for resistance to a Korean isolate of rice blast. Rice Genet Newsl 13: 74–76.
Ahn, S.N., Y.K. Kim, H.C. Hong, S.S. Han, H.C. Choi, H.P. Moon & S.R. McCouch, 1996b. Molecular mapping of genes for resistance to major Korean isolates of rice blast (in Korean). Korean J Breed 29(4): 416–423.
Causse, M.A., T.M. Fulton, Y.G. Cho, S.N. Ahn, J. Chunwongse, K.S. Wu, J.H. Xiao, Z.H. Yu, P.C. Ronald, S.E. Harrington, G. Second, S.R. McCouch & S.D. Tanksley, 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 1251–1274.
Chen, X., S. Temnykh, Y. Xu, Y.G. Cho & S.R. McCouch, 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor Appl Genet 95: 553–567.
Ikehashi, I. & G.S. Khush, 1979. Breeding for blast resistance at IRRI. In: Proceedings of the Rice Blast Workshop, pp. 69–80. IRRI, Manila, Philippines.
Inukai, T., R.J. Nelson, R.S. Zeigler, S. Sarkarung, D.J. Mackill, J.M. Bonman, I. Takamure & T. Kinoshita, 1994. Allelism of blast resistance genes in near-isogenic lines of rice. Phytopathology 84: 1278–1283.
Kim, Y.K., Y.J. Kim, H.C. Choi & S.S. Han, 1995. Inheritance of leaf blast reactions to four races of blast fungus in segregating populations of eight rice crosses (in Korean). RDA J Agri Sci 37: 283–298.
Kiyosawa, S., H. Ikehashi, H. Kato & Z.Z. Ling, 1981. Pathogenicity tests of Philippine isolates of blast fungus using two sets of rice varieties.Japan J Breed31: 367–376.
Kiyosawa, S., 1969. Inheritance of resistance of rice varieties to a Philippine fungus strain of Pyricularia oryzae. Japan J Breed 19:61–73.
Kosambi, D.D., 1944. The estimation of map distance from recombination values. Ann Eugen 12: 172–175.
Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary linkage maps of experimental and natural populations. Genomics 1: 174–181.
Lee, E.J. & S.Y. Cho, 1990. Variation in races of rice blast disease and varietal resistance in Korea. Paper presented at the Focus on Irrigated Rice, August 1990, pp. 27-31. Seoul, Korea.
Lee, E.J., J.D. Ryu, W.H. Yeh, S.S. Han & Y.H. Lee YH, 1987. Proposal of a new method for differentiating pathogenic races of Pyricularia oryzae Cavara in Korea (in Korean). RDA J Agri Sci (PM & U) 29: 206–213.
Maheswaran, M., P.K. Subudhi, S. Nandi, J.C. Xu, A. Parco, D.C. Yang & N. Huang, 1996. Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population. Theor Appl Genet 94: 39–45.
McCouch, S.R., R.J. Nelson, J. Thome & R.S. Zeigler, 1994. Mapping of blast resistance genes in rice. In: R.S. Zeigler, S.A. Leong & P.S. Teng (Eds.), Rice Blast Disease, pp. 167–186. Cab Int'l and IRRI, Wallingford, Oxon, UK.
McCouch, S.R. & S.D. Tanksley, 1991. Development and use of restriction fragment length polymorphism in rice breeding and genetics. In: G.S. Khush & G.H. Toenniessen (Eds.), Rice Biotechnology, pp. 109–133. Cab Int'l and IRRI, Wallingford, Oxon, UK.
Mew, T.V., A.S. Parco, S. Hittalmani, T. Inukai, R. Nelson, R.S. Zeigler & N. Huang, 1994. Fine-mapping of major genes for blast resistance in rice. Rice Genet Newsl 11: 126–128.
National Crop Experiment Station, 1996. Annual Report (in Korean), pp. 33-36.
Naqvi, N.I., M. Bonman, D.J. Mackill, R.J. Nelson & B.B. Chattoo, 1995. Identification of RAPD markers linked to a major blast resistance gene in rice. Mol Breed 1: 341–348.
Panaud, O., X.L. Chen & S.R. McCouch, 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252: 597–607.
Ryu, J.D., W.H. Yeh, S.S. Han, Y.H. Lee & E.J. Lee, 1987. Regional and annual fluctuation of races of Pyricularia oryzae during 1977-1985 in Korea (in Korean). Korean J Plant Path 3(3): 174–179.
Saito, A., M. Yano, N. Kishimoto, M. Nakagahra, A. Yoshimura, K. Saito, S. Kuhara, Y. Ukai, M. Kawase, T. Nagamine, S. Yoshimura, O. Ideta, R. Ohsawa, Y. Hayano, N. Iwata & M. Sugira, 1991. Linkage map of restriction fragment length polymorphism loci in rice. Jap J Breed 41: 665–670.
Song, W.Y., G.L. Wang, L. Chen, H.S. Kim, B. Wang, T. Holsten, W.X. Zhai, L.H.C. Fauquet & P. Ronald, 1995. The rice disease resistance gene Xa-21 encodes a receptor kinase-like protein. Science 70: 1804–1806.
Wang, G., D.J. Mackill, J.M. Bonman, S.R. McCouch, M. Champoux & R.J. Nelson, 1994. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136: 1421–1434.
Yu, Z.H., D.J. Mackill, J.M. Bonman, S.R. McCouch, E. Guiderdoni, J.L. Notteghem & S.D. Tanksley, 1996. Molecular mapping of genes for resistance to rice blast (Pyricularia grisea Sacc.). Theor Appl Genet 93: 859–863.
Yu, Z.H., D.J. Mackill, J.M. Bonman & S.D. Tanksley, 1991. Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81: 471–476.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ahn, SN., Kim, YK., Hong, HC. et al. Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.). Euphytica 116, 17–22 (2000). https://doi.org/10.1023/A:1004045812357
Issue Date:
DOI: https://doi.org/10.1023/A:1004045812357