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Metal-humic complexes and plant micronutrient uptake: a study based on different plant species cultivated in diverse soil types

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Abstract

There are several studies in the literature dealing with the effect of metal-humic complexes on plant metal uptake, but none of them correlate the physicochemical properties of the complexes with agronomic results. Our study covers both aspects under various experimental conditions. A humic extract (SHE) obtained from a sapric peat was selected for preparing the metal–humic complexes used in plant experiments. Fe–, Zn– and Cu–humic complexes with a reaction stoichiometry of 2:0.25 (humic:metal, w/w) were chosen after studying their stability and solubility with respect to pH (6–9) and the humic:metal reaction stoichiometry. Wheat and alfalfa plants were greenhouse cultured in pots containing one of three model soils: an acid, sandy soil and two alkaline, calcareous soils. Treatments were: control (no additions), SHE (53 mg kg−1 of SHE), and metal (Cu, Zn and Fe)–SHE complexes (2.5 and 5 mg kg−1 of metal rate and a SHE concentration to make 53 mg kg −1). Cu- and Zn–humic complexes significantly (p≤0.05) increased the plant uptake and the DTPA-extractable soil fraction of complexed micronutrients in most plant–soil systems. However, these effects were associated with significant increases (p≤0.05) of shoot and root dry weight only in alfalfa plants. In wheat, significant increases of root and shoot dry matter were only observed in the Cu–humic treated plants growing in the acid soil, where Cu deficiency was more intense. The Fe–humic complex did not increase Fe plant assimilation in any plant–soil system, but SHE increased Fe-uptake and/or DTPA-extractable soil Fe in the wheat–calcareous soil systems. These results, taken together with those obtained from the study of the pH- and SHE:metal ratio-dependent SHE complex solubility and stability, highlight the importance of the humic:Fe complex stoichiometry on iron bioavailability as a result of its influence on complex solubility.

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Authors and Affiliations

  1. R&D Department, Inabonos-Roullier Group, Spain

    J.M. García-Mina

  2. Department of Chemistry and Soil Chemistry, Faculty of Sciences, University of Navarra, PO Box 273, 31080, Pamplona (Navarra), Spain

    J.M. García-Mina

  3. Department of Plant Physiology, Faculty of Sciences, University of Navarra, PO Box 273, 31080, Pamplona (Navarra), Spain

    M.C. Antolín & M. Sanchez-Diaz

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  1. J.M. García-Mina
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  2. M.C. Antolín
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  3. M. Sanchez-Diaz
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García-Mina, J., Antolín, M. & Sanchez-Diaz, M. Metal-humic complexes and plant micronutrient uptake: a study based on different plant species cultivated in diverse soil types. Plant and Soil 258, 57–68 (2004). https://doi.org/10.1023/B:PLSO.0000016509.56780.40

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