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Dietary methionine requirement of fingerling Indian major carp, Cirrhinus mrigala (Hamilton)

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Abstract

Indian major carp, Cirrhinus mrigala fingerling (3.85 ± 0.50 cm, 0.50 ± 0.02 g) were fed isonitrogenous and isocaloric diets (40% CP, 4.28 kcal g−1, GE) containing casein, gelatin and crystalline amino acids with graded levels of L- methionine (0.50, 0.75, 1.00, 1.25, 1.50 and 2.00 g/ 100 g, dry diet) with 1.00% cystine fixed, to determine its dietary methionine requirement. A feeding trial was conducted in triplicate for six weeks. Diets were fed twice a day at 0800 and 1600 h at 5% of body weight/day. The ration size and feeding regime were worked out prior to the start of the feeding trial. Weight gain (158%) and food conversion ratio (1.45) were significantly (P < 0.05) higher in fish fed diet containing 1.00% methionine with 1.00% cystine fixed. Second degree polynomial regression analysis of the weight gain data indicated the dietary methionine requirement to be 1.20 g/100 g of dry diet, corresponding to 3.00% of dietary protein. Second degree polynomial regression analysis was also employed to determine the relationship between food conversion ratio (FCR) and dietary methionine levels which indicated that the best FCR occurred at approximately 1.20% dietary methionine level. Carcass composition of fish fed diet containing graded levels of methionine varied significantly (P < 0.05) except carcass ash content which showed insignificant (P > 0.05) differences among the dietary methionine levels.

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  1. Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, 202 002, India

    Imtiaz Ahmed, Mukhtar A. Khan & A.K. Jafri

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  1. Imtiaz Ahmed
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  2. Mukhtar A. Khan
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  3. A.K. Jafri
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Correspondence to Mukhtar A. Khan.

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Ahmed, I., Khan, M.A. & Jafri, A. Dietary methionine requirement of fingerling Indian major carp, Cirrhinus mrigala (Hamilton). Aquaculture International 11, 449–462 (2003). https://doi.org/10.1023/B:AQUI.0000004181.89420.a2

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