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The Activity of the Antioxidative System in Cadmium-Treated Arabidopsis thaliana

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Biologia Plantarum

Abstract

Changes in the content of reactive oxygen species (ROS) and activity of the antioxidant system were measured in leaves of Arabidopsis thaliana (L.) Heynh exposed to Cd2+. Mature plants growing in the nutrient solution were treated with Cd2+ at different concentrations (0, 5, 25, 50, 100 μM). An increase of \({\text{O}}_{\text{2}}^{ \bullet {\text{ - }}} \) content in leaves was observed at 5, 25 and 50 μM Cd2+. A strong accumulation of H2O2 was found only at the lowest Cd2+ concentration. The content of OH*. was high at 50 and 100 μM Cd2+. Superoxide dismutase (SOD) activity was always higher in Cd2+-treated plants than in control. Catalase (CAT) activity decreased with increasing Cd2+ concentration in the nutrient solution. Guaiacol peroxidase (POX) activity was particularly high at the lowest and highest Cd2+ concentrations and ascorbate peroxidase (APX) activity additionally at 50 μM Cd2+. Enhanced activity of monodehydroascorbate reductase (MDHAR) and strong reduction in ascorbate (AA) content were observed at 25 μM Cd2+. Glutathione reductase (GR) activity was always higher than in control but decreased as Cd2+ concentration increased. However, it was accompanied by gradual content increase of SH-groups.

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Authors and Affiliations

  1. Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, PL-20033, Lublin, Poland

    E. Skórzyńska-Polit, M. Dra¸żkiewicz & Z. Krupa

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  1. E. Skórzyńska-Polit
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  2. M. Dra¸żkiewicz
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  3. Z. Krupa
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Correspondence to E. Skórzyńska-Polit.

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Skórzyńska-Polit, E., Dra¸żkiewicz, M. & Krupa, Z. The Activity of the Antioxidative System in Cadmium-Treated Arabidopsis thaliana . Biologia Plantarum 47, 71–78 (2003). https://doi.org/10.1023/A:1027332915500

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