Skip to main content
Log in

The Effects of Growth Regulators on Flowering of Chenopodium murale Plants in vitro

Biologia Plantarum

Abstract

In vitro culture of Chenopodium murale L. (ecotype 197) green and herbicide SAN 9789 - treated "white" plants was established and the effects of benzylaminopurine (BAP), indole-3-acetic acid (IAA) and gibberellic acid (GA3) on growth and flowering were tested. Green plants did not flower on glucose free media, while 17 % of plants flowered on 5 % glucose-containing medium. SAN 9789 (10−5 M) inhibited growth and flowering. BAP and IAA (0.1 – 5 mg dm−3) also inhibited growth and flowering of green and "white" plants. GA3 (10 mg dm−3) stimulated leaf development in green plants, but had no significant effect on "white" plants, and stimulated flowering of green (41 %) and "white" (33 %) plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bernier, G., Kinet, J.-M., Jacquard, A., Havelange, A., Bodson, M.: Cytokinin as a possible component of the floral stimulus in Sinapis alba-Plant Physiol. 60: 282-285, 1977.

    Google Scholar 

  • Chailakhyan, M.Kh.: Hormonale Faktoren des Pflanzenblühens.-Biol. Zentralbl. 77: 641-662, 1958.

    Google Scholar 

  • Ćulafić, Lj., Konjević, R., Nešković, M.: Flowering in vitro grown spinach shoots in the presence of the herbicide Sandoz 9789.-Biol. Plant. 25: 155-157, 1983.

    Google Scholar 

  • Evans, L.T., King, R.W., Mander, L.N., Pharis, R.P.: The relative significance for stem elongation and flowering in Lolium temulentum of 3β-hydroxylation of gibberellins.-Planta 192: 130-136, 1994.

    Google Scholar 

  • Havelange, A., Bodson, M., Bernier, B.: Partial floral evocation by exogenous cytokinin in the long-day plant Sinapis alba.-Physiol. Plant. 67: 695-701, 1986.

    Google Scholar 

  • Hilton, J.R., Smith, H.: The presence of phytochrome in purified barley etioplasts and its in vitro regulation of biologically-active gibberellin levels in etioplasts.-Planta 148: 312-318, 1980.

    Google Scholar 

  • Krekule, J., Pavlová, L., Součková, D., Macháčková, I.: Auxin in flowering of short-day and long-day Chenopodium species.-Biol. Plant. 27: 310-317, 1985.

    Google Scholar 

  • Mitrović, A.: In vitro flowering of Chenopodium rubrum L., a short-day plant and Chenopodium murale L., a long-day plant.-Thesis. University of Belgrade, Belgrade 1998.

    Google Scholar 

  • Mitrović, A., živanović, B., Ćulafić, L.: The effect of photoperiod, glucose and gibberellic acid on growth in vitro and flowering of Chenopodium murale.-Biol. Plant. 43: 173-177, 2000.

    Google Scholar 

  • Pavlová, L., Krekule, J.: Fluctuation of free IAA under inductive and non-inductive photoperiods in Chenopodium rubrum.-J. Plant Growth Regul. 2: 94-98, 1984.

    Google Scholar 

  • Podolny, V.Z., Macháčková, I., Josefusová-Vondráková, Z., Eder, J., Krekule, J., Chailakhyan, M.Kh.: The role of indol-3-ylcetic acid in regulation of juvenility in Xanthium strumarium L.-Biol. Plant. 33: 26-31, 1991.

    Google Scholar 

  • Rappaport, L., Adams, D.: Gibberelins: synthesis, compartmentation and physiological process.-Phil. Trans. roy. Soc. London B 284: 521-539, 1979.

    Google Scholar 

  • Scorza, R.: In vitro flowering.-Hort. Rev. 4: 106-127, 1982.

    Google Scholar 

  • Seidlová, F.: Growth regulators in changing apical growth at transition to flowering.-Biol. Plant. 27: 350-359, 1985.

    Google Scholar 

  • Vondráková, Z.: The effect of 6-benzylaminopurine on in vitro flowering of Chenopodium rubrum.-In: Kamínek, M., Mok, D.W.S., Zažímalová, E. (ed.): Physiology and Biochemistry of Cytokinins in Plants. Pp. 385-387, SPB Academic Publishin, The Hague 1992.

    Google Scholar 

  • Vondráková, Z., Krekule, J., Macháčková, I.: Is the root effect on flowering of Chenopodium rubrum mediated by cytokinins?-J. Plant Growth Regul. 17: 115-119, 1998.

    Google Scholar 

  • Wagner, E., Leonhard, J.: Photoperiodische Induktion der Blutenbildung bei Kurtzag-pflanze Chenopodium rubrum L.-Biol. uns. Zeit. 4: 120-125, 1985.

    Google Scholar 

  • živanović, B., Ćulafić, Lj.: Photoperiodic induction of flowering in green and photobleached Chenopodium rubrum L. ecotype 184 —a short-day plant.-Biol. Plant. 34: 457-460, 1992.

    Google Scholar 

  • živanović, B., Ćulafić, Lj., Filipović, A.: The effects of hormones and saccharides on growth and flowering of green and herbicides-treated Chenopodium rubrum L. plants.-Biol. Plant. 37: 257-264, 1995.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. Mitrović.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mitrović, A., Živanović, B. & Ćulafić, L. The Effects of Growth Regulators on Flowering of Chenopodium murale Plants in vitro. Biologia Plantarum 43, 451–454 (2000). https://doi.org/10.1023/A:1026799906616

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026799906616

Navigation