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Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis

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Abstract

Three sulfated polysaccharide fractions (F1, F2, and F3) were isolated from Porphyra haitanesis, an important economic alga in China, through anion-exchange column chromatography and their in vitro antioxidant activities were investigated in this study. Galactose was the main sugar unit of the three fractions. The analytical results indicated that polysaccharide fractions from P. haitanesis had similar chemical components to porphyran from other species, but differed in their high sulfate content. The sulfate content of F1, F2 and F3 was 17.4%, 20.5% and 33.5% respectively. All three polysaccharide fractions showed antioxidant activities. They had strong scavenging effect on superoxide radical, and much weaker effect on hydroxyl free radical. Lipid peroxide in rat liver microsome was significantly inhibited, and H2O2 induced hemolysis of rat erythrocyte was partly inhibited by F1, F2 and F3. Among them, F3 showed strongest scavenging effect on superoxide radical; F2 had strongest effect on hydroxyl radical and lipid peroxide.

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References

  • Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 7: 248-254.

    Google Scholar 

  • Brasch D.J., Chang H.M., Chuah C.T. and Melton L.D. 1981. The galactan sulphate from the edible red alga Porphyra columbina. Carbohydr. Res. 97: 113-125.

    Google Scholar 

  • Cuzzocrea S., Riley D., Caputi A.P. and Selvemini D. 2001. Antioxidant therapy: A new pharmacological approach in shock, in-flammation, and ischemia/reperfusion injury. Pharmacol. Revs. 53: 135-159.

    Google Scholar 

  • Dubois M., Gillis K.A., Hamilton J.K., Rebers P.A. and Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356.

    Google Scholar 

  • Haroun-Bouhedia F., Ellouali M., Sinquin C. and Boisson-Videl C. 2000. Relationship between sulfate groups and biological activities of fucans. Thromb. Res. 100: 453-459.

    Google Scholar 

  • Hu J.F., Geng M.Y., Zhang J.T. and Jiang H.D. 2001. An in vitro study of the structure-activity relations of sulfated polysaccharide from brown algae to its antioxidant effect. J. Asian Nat. Prod. Reports 3: 353-358.

    Google Scholar 

  • Kawai Y. 1969. Modified methods to determine sulfate in polysaccharide. Anal. Chem. 32: 314- 321.

    Google Scholar 

  • Li Z.X., Huang C.G., Cai Y.J., Chen X.M., Wang F. and Chen Y.Z. 2000. The chemical structure and antioxidative activity of polysaccharide from Asparagus cochinchinensis. Acta Pharma. Sin. 35: 358-362, (in Chinese).

    Google Scholar 

  • Liu F., Ooi V.E. and Chang S.T. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sciences 60: 763-771.

    Google Scholar 

  • Nishide E., Ohno M., Anzai H. and Uchida N. 1988. Extraction of porphyran from Porphyra yezonesis Udea F. narawaensis MIURA. Nippon Suisan Gakkaishi 54: 2189-2194, (in Japanese).

    Google Scholar 

  • Percival E. 1979. The polysaccharides of green, red and brown seaweeds: their basic structure, biosynthesis and function. Br. phycol. J. 14: 103-117.

    Google Scholar 

  • Ponti V., Dianzani M.V. and Cheeseman K.J. 1978. Studies on the reduction of nitroblue tetrazolium chloride mediated through the action of NADH and phenazine methosulate. Chem. Biol. Interact. 23: 281-285.

    Google Scholar 

  • Ruperez P., Ahrazem O. and Leal J.A. 2002. Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J. Agric. Food Chem. 50: 840-845.

    Google Scholar 

  • Shi S.Y. 1991. Porphyran and its chemistry. Chinese Journal of Marine Drugs 10: 21-26, (in Chinese).

    Google Scholar 

  • Smirnoff N. and Cumbes Q.J. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28: 1057-1060.

    Google Scholar 

  • Tsipali E., Whaley S., Kalbfleisch J., Ensley H.E., Browder I.W. and Williams D.L. 2001. Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity. Free Radical Biology and Medicine 30: 393-402.

    Google Scholar 

  • Xue C., Fang Y., Lin H., Chen L., Li Z., Deng D. et al. 2001. Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica. J. appl. Phycol. 13: 67-70.

    Google Scholar 

  • Yaphe W. and Arsenault G.P. 1965. Improved resornol reagent for the determination of fructose and 3,6-anhydrogalactose in polysaccharides. Anal. Biochem. 3: 143-148.

    Google Scholar 

  • Yashizawa Y., Enomoto A., Todoh H., Ametani A. and Kaminogawa S. 1993. Activation of murine macrophages by polysaccharide fractions from marine alga (Porphyra yezoensis). Biosci. Biotech. Biochem. 57: 1862-1866.

    Google Scholar 

  • Yashizawa Y., Ametani A., Tsunehiro J., Numura K., Itoh M., Fukui F. et al. 1995. Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure-function relationships and improved solubility. Biosci. Biotech. Biochem. 59: 1933-1937.

    Google Scholar 

  • Zhou H.P. and Chen Q.H. 1990. Anticoagulant and antihyperlipedemic effects of polysaccharide from Porphyra Yezoensis. J. China Pharm. Univ. 21: 358-360, (in Chinese).

    Google Scholar 

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Zhang, Q., Yu, P., Li, Z. et al. Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis . Journal of Applied Phycology 15, 305–310 (2003). https://doi.org/10.1023/A:1025137728525

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