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Divinyl Sulfone as a Crosslinking Reagent for Oligomeric Proteins

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Abstract

The kinetics of the nucleophilic addition reactions of divinyl sulfone to amino groups of glycine and model proteins was studied in aqueous solution at 30°C. The rate constants for glycine, bovine serum albumin, and α1-casein were (4.84 ± 0.58) × 10–1, (2.97 ± 0.31) × 10–2, and (2.38 ± 0.49) × 10–2 M–1 s–1, respectively. Divinyl sulfone was proposed as a crosslinking reagent for the qualitative detection of protein association in solution. The crosslinking capacity of divinyl sulfone was compared to that of 1,3,5-triacryloylhexahydro-s-triazine.

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Authors and Affiliations

  1. Faculty of Fundamental Sciences, Gediminas Technical University, Vilnius, Sauletekio al. 11, Vilnius, 2040, Lithuania

    J. Sereikaitė, D. Bassus, Zh. Bumelienė & V.-A. Bumelis

  2. Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius, 2006, Lithuania

    R. Bobnis & G. Dienys

Authors
  1. J. Sereikaitė
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  2. D. Bassus
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  3. R. Bobnis
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  4. G. Dienys
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  5. Zh. Bumelienė
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  6. V.-A. Bumelis
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Correspondence to J. Sereikaitė.

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Sereikaitė, J., Bassus, D., Bobnis, R. et al. Divinyl Sulfone as a Crosslinking Reagent for Oligomeric Proteins. Russian Journal of Bioorganic Chemistry 29, 227–230 (2003). https://doi.org/10.1023/A:1023928314772

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