Abstract
The temporal pattern of pheromone emission by Anaphes listronoti females was established in a four-arm olfactometer by observing, at 2-hr intervals, the response of <2-hr-old males to odor produced by individual virgin females of <1, 1, and 2 days of age. This is the first evidence of a sexual pheromone in a Mymaridae. Under a 16L:8D photoperiod and 24°C constant temperature, the responsiveness of males to females of different ages varied significantly during the photophase. When <1-day-old females were used, males made significantly more final choices in the pheromone field than odorless fields at 4 and 6 hr after the onset of the photophase, and their walking speed was significantly higher from 4 to 12 hr, suggesting that females began to release a long-range pheromone during this period. Final choices and high walking speed were observed earlier with 1- and 2-day-old females than with <1-day-old females, and there was a significant decrease in male responses at 6 hr after the onset of the photophase, suggesting a bimodal temporal pattern of sexual pheromone emission.
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REFERENCES
Assem, J., van den. 1986. Mating behaviour in parasitic wasps, pp. 137–167, in J. Waage and D. Greathead (eds.). Insect Parasitoids. Academic Press, Orlando, Florida.
Assem, J. van den, and Jachmann, F. 1982. The coevolution of receptivity signalling and body size dimorphism in the Chalcidoidea. Behaviour 80:96–105.
Assem, J. van den, and Putters, F. A. 1980. Patterns of sound produced by courting chalcidoid males and its biological significance. Entomol. Exp. Appl. 27:293–302.
Baker, T. C., Meyer, W., and Roelofs, W. L. 1981. Sex pheromone dosage and blend specificity of response by oriental fruit moth males. Entomol. Exp. Appl. 30:269–279.
Bakkendorf, O. 1934. Biological investigations on some Danish hymenopterous egg-parasites, especially in homopterous and heteropterous eggs, with taxonomic remarks and descriptions of new species. Entomol. Medd. 19:1–134.
Bartelt, R. J., Jones, R. L., and Kulman, H. M. 1982. Hydrocarbon components of the yellow-headed spruce sawfly sex pheromone: A series of (Z,Z)-9, 19-dienes. J. Chem. Ecol. 8:95–114.
Berry, J. S., and Holtzer, T. O. 1990. Ambulatory dispersal behavior of Neoseiulus fallacis (Acarina: Phytoseiidae) in relation to prey density and temperature. Exp. Appl. Acarol. 8:253–274.
Bigler, F., Bieri, M., Fritschy, A., and Seidel, K. 1988. Variation in locomotion between laboratory strains of Trichogramma maidis and its impact on parasitism of eggs of Ostrinia nubilalis in the field. Entomol. Exp. Appl. 49:283–290.
Boivin, G. 1988. Laboratory rearing of Anaphes soridatus (Hym.: Mymaridae) on carrot weevil eggs (Col.: Curculionidae). Entomophaga 33:245–248.
Brodeur, J., and McNeil, J. N. 1994. Seasonal ecology of Aphidius nigripes (Hymenoptera: Aphidiidae), a parasitoid of Macrosiphum euphorbiae (Homoptera: Aphididae). Environ. Entomol. 23:292–298.
Clausen, C. P. 1940. Entomophagous Insects. McGraw-Hill, New York.
Collins, R. D. 1982. The biology of Patasson sp. near sordidatus (Hymenoptera: Mymaridae) and its impact on the carrot weevil. MS thesis. Michigan State University, East Lansing.
Collins, R. D., and Grafius, E. 1986a. Biology and life cycle of Anaphes sordidatus (Hymenoptera: Mymaridae), an egg parasitoid of the carrot weevil (Coleoptera: Curculionidae). Environ. Entomol. 15:100–105.
Collins, R. D., and Grafius, E. 1986b. Impact of the egg parasitoid, Anaphes sordidatus (Hymenoptera: Mymaridae), on the carrot weevil (Coleoptera: Curculionidae). Environ. Entomol. 15:469–475.
Collins, R. D., and Grafius, E. 1986c. Courtship and mating behavior of Anaphes sordidatus (Hymenoptera: Mymaridae), a parasitoid of the carrot weevil (Coleoptera: Curculionidae). Ann. Entomol. Soc. Am. 79:31–33.
Cormier, D., Stevenson, A. B., and Boivin, G. 1996. Seasonal ecology and geographical distribution of Anaphes listronoti and A. victus (Hymenoptera: Mymaridae), egg parasitoids of the carrot weevil (Coleoptera: Curculionidae) in central Ontario. Environ. Entomol. 25:1376–1382.
Daniel, W. W. 1990. Applied Nonparametric Statistics. PWS-Kent Publishing, Boston.
Decker, U. M., Powell, W., and Clark, S. J. 1993. Sex pheromones in the cereal aphid parasitoids Praon volucre and Aphidius rhopalosiphi. Entomol. Exp. Appl. 69:33–39.
Doutt, R. L. 1959. The biology of parasitic hymenoptera. Annu. Rev. Entomol. 4:161–182.
Giles, D. K., Heinz, K. M., and Parrella, M. P. 1996. Quantitative assessment of insect olfactometer performance by experimental flow analysis. Biol. Control 7:44–47.
Godray, H. C. J. 1994. Parasitoids: Behavioral and Evolutionary Ecology. Princeton University Press, Princeton, New Jersey.
Gordh, G., and DeBach, P. 1978. Courtship behavior in the Aphytis lingnanensis group, its potential usefulness in taxonomy, and a review of sexual behavior in the parasitic Hymenoptera (Chalcidoidea: Aphelinidae). Hilgardia 46:37–75.
Haynes, K. F., Gaston, L. K., Pope, M. M., and Baker, T. C. 1983. Rate and periodicity of pheromone release from individual female artichoke plume moths, Platyptilia carduidactyla (Lepidoptera: Pterophoridae). Environ. Entomol. 12:1597–1600.
Kimani, S. W., and Overholt, W. A. 1995. Biosystematics of the Cotesia flavipes complex (Hymenoptera: Braconidae): Interspecific hybridization, sex pheromone and mating behaviour studies. Bull. Entomol. Res. 85:379–386.
Leonard, S. H., and Ringo, J. M. 1978. Analysis of male courtship patterns and mating behavior of Brachymeria intermedia. Ann. Entomol. Soc. Am. 71:817–826.
Martel, P., Svec, H. J., and Harris, C. R. 1975. Mass rearing of the carrot weevil, Listronotus oregonensis (Coleoptera: Curculionidae), under controlled environmental conditions. Can. Entomol. 107:95–98.
McNeil, J. N., and Brodeur, J. 1995. Pheromone-mediated mating in the aphid parasitoid, Aphidius nigripes (Hymenoptera: Aphididae). J. Chem. Ecol. 21:959–972.
Pettersson, J. 1970. An aphid sex attractant. I. Biological studies. Entomol. Scand. 1:63–73.
Pintureau, B., and Toonders, T. 1983. Quelques résultats concernant l'étude de l'attraction des mâles par les femelles vierges chez Trichogramma maidis (Hym. Trichogrammatidae). Bull. Soc. Linn. Lyon 52:81–87.
Pittara, I. S., and Katsoyannos, B. I. 1985. Male attraction to virgin females in the almond seed wasp, Eurytoma amygdali Enderlein (Hymenoptera, Eurytomidae). Entomol. Hell. 3:43–46.
Raina, A. K., Klun, J. A., and Stadelbacher, E. A. 1986. Diel periodicity and effect of age and mating on female sex pheromone titer in Heliothis zea (Lepidoptera: Noctuidae). Ann. Entomol. Soc. Am. 79:128–131.
Rao, S. V., and DeBach, P. 1969. Experimental studies on hybridization and sexual isolation between some Aphytis species (Hymenoptera: Aphelinidae). I. Experimental hybridization and an interpretation of evolutionary relationships among the species. Hilgardia 39:515–553.
Read, D. P., Feeny, P. P., and Root, R. B. 1970. Habitat selection by the aphid parasite Diaeretiella rapae (Hymenoptera: Braconidae) and hyperparasite Charips brassicae (Hymenoptera: Cynipidae). Can. Entomol. 102:1567–1578.
Reed, H. C., Tan, S., Reed, D. K., Elliott, N. C., Burd, J. D., and Walker, T. 1994. Evidence for a sex attractant in Aphidius colemani Viereck with potential use in field studies. Southwest Entomol. 19:273–278.
Robacker, D. C., Weaver, K. M., and Hendry, L. B. 1976. Sexual communication and associative learning in the parasitic wasp Itoplectis conquisitor (Say). J. Chem. Ecol. 2:39–48.
SAS Institute. 1988. SAS/STAT User's Guide, Release 6.03 ed. SAS Institute, Cary, North Carolina.
Schwartz, A., and Gerling, D. 1974. Adult biology of Telenomus remus (Hymenoptera: Scelionidae) under laboratory conditions. Entomophaga 19:483–492.
Steel, R. G. D., and Torrie, J. H. 1980. Principles and Procedures of Statistics: A Biometrical Approach. McGraw-Hill, New York.
Swedenborg, P. D., and Jones, R. L. 1992a. Multicomponent sex pheromone in Macrocentrus grandii Goidanich (Hymenoptera: Braconidae). J. Chem. Ecol. 18:1901–1912.
Swedenborg, P. D., and Jones, R. L. 1992b. (Z)-4-Tridecenal, a pheromonally active air oxidation product from a series of (Z,Z)-9,13 dienes in Macrocentrus grandii Goidanich (Hymenoptera: Braconidae). J. Chem. Ecol. 18:1913–1931.
Swedenborg, P. D., Jones, R. L., Liu, H.-W., and Krick, T. P. 1993. (3R*,5S*,6R*s*)-3,5-Dimethyl-6-(methylethyl)-3,4,5,6-tetrahydropyran-2-one, third sex pheromone component for Macrocentrus grandii (Goidanich) (Hymenoptera: Braconidae) and evidence for its utility at eclosion. J. Chem. Ecol. 19:485–502.
Swier, S. R., Rings, R. W., and Musick, G. J. 1977. Age-related calling behavior of the black cutworm, Agrotis ipsilon. Ann. Entomol. Soc. Am. 70:919–924.
Takahashi, S., and Sugai, T. 1982. Mating behavior of the parasitoid wasp Tetrastichus hagenowii (Hymenoptera: Eulophidae). Entomol. Gen. 7:287–293.
Tripathi, R. N., and Singh, R. 1990. Mating behaviour of Lysiphlebia mirzai Shuja-Uddin (Hymenoptera: Aphidiidae), a parasitoid of Rhopalosiphum maidis (Fitch) (Hemiptera: Aphididae). Entomon 15:21–25.
Vet, L. E. M., van Lenteren, J. C., Heymans, M., and Meelis, E. 1983. An airflow olfactometer for measuring olfactory responses of hymenopterous parasitoids and other small insects. Physiol. Entomol. 8:97–106.
Vigneault, C., Panneton, B., Cormier, D., and Boivin, G. 1997. Automated system to quantify the behavior of small insects in a four-pointed star olfactometer. Appl. Eng. Agric. 13:545–550.
Vinson, S. B. 1972. Courtship behavior and evidence for a sex pheromone in the parasitoid Campoletis sonorensis (Hymenoptera: Ichneumonidae). Environ. Entomol. 1:409–414.
Whitcomb, W. D. 1965. The carrot weevil in Massachusetts; biology and control. Agriculture Experiment Station, University of Massachusetts Bulletin No. 550.
Yoshida, S. 1978. Behaviour of males in relation to the female sex pheromone in the parasitoid wasp, Anisopteromalus calandrae (Hymenoptera: Pteromalidae). Entomol. Exp. Appl. 23:152–162.
Yoshida, S., and Hidaka, T. 1979. Determination of the position of courtship display of the young unmated male Anisopteromalus calandrae. Entomol. Exp. Appl. 26:115–120.
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Cormier, D., Royer, L., Vigneault, C. et al. Effect of Female Age on Daily Cycle of Sexual Pheromone Emission in Gregarious Egg Parasitoid Anaphes listronoti . J Chem Ecol 24, 1595–1610 (1998). https://doi.org/10.1023/A:1020860326945
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DOI: https://doi.org/10.1023/A:1020860326945