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Differential Effect of Nitric Oxide Inhibition as a Function of Preservation Period in Pancreas Transplantation

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Abstract

The role of nitric oxide, produced duringreperfusion as a function of preservation time, in thedevelopment of the inflammatory process in pancreastransplantation has been explored. For this purpose, the effect of nitric oxide synthase inhibition,as well as 6-keto-prostaglandin F1α,leukotriene B4, and lipoperoxidation levelswere evaluated in an experimental model of rat pancreastransplantation after different periods of cold preservation.The results show posttransplantation increases in6-keto-prostaglandin F1α, leukotrieneB4, and lipoperoxidation levels in pancreatictissue and in plasma lipase. When ischemia was induced for 30 min, nitricoxide synthase inhibition prevented these increases, andL-arginine was able to reverse this effect. By contrast,nitric oxide synthase inhibition has no effect when ischemia was prolonged for 12 hr. Insummary, this study suggests that, during reperfusion,nitric oxide modulates 6-keto-prostaglandinF1α synthesis, lipoperoxidation levels,and the development of pancreatic injury but only whenthe ischemic period is quite short.

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Authors
  1. Laureano Fernandez-Cruz
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  2. Francisco Badosa
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  3. Emilio Gelpi
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  4. Felip Pi
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  5. Georgina Hotter
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  6. Daniel Closa
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  7. Neus Prats
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  8. Joan Rosello-Catafau
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Fernandez-Cruz, L., Badosa, F., Gelpi, E. et al. Differential Effect of Nitric Oxide Inhibition as a Function of Preservation Period in Pancreas Transplantation. Dig Dis Sci 42, 962–971 (1997). https://doi.org/10.1023/A:1018824700470

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