Abstract
Sequence-specific-oligonucleotides analysis has been used to identify Dunaliella bardawil, D. salina and D. parva from hypersaline environments based on their structural features of introns from the 18S rDNA. Carotenogenic and halophilic strains such as D. bardawil and D. salina were identified as harboring II and I introns within 18S rDNA, respectively. This is the first report on the existence of D. bardawil in saline water bodies of Mexico and Latin America.
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References
Aasen AJ, Eimehjellen KE, Liaaen-Jensen S (1969) An extreme source of ?-carotene. Acta Chem. Scand. 23: 2544.
Becker EW (1995) Microalgae in Biotechnology and Microbiology, Cambridge: Cambridge University Press.
Ben-Amotz A, Avron M (1982) The potential use of Dunaliella for the production of glycerol, ?-carotene and high-protein feed. In: San Pietro A. ed., Biosaline Research: A Look to the Future. New York: Plenum Publishing Corporation, pp. 207-214.
Ben-Amotz A, Avron M (1983) On the factors which determine the massive ?-carotene accumulation in the halotolerant alga Dunaliella bardawil. Plant Physiol. 72: 593-597.
Borowitzka MA, Borowitzka LJ (1988) Micro-Algal Biotechnology. Cambridge: Cambridge University Press.
Buchheim MA, Lemieux C, Otis Ch, Gutell R, Chapman RL, Turmel M (1996) Phylogeny of the Chlamydomonales (Chlorophyceae): a comparison of ribosomal RNA gene sequences from the nucleus and the chloroplast. Mol. Phyl. Evol. 5: 391-402.
DeLong EF, Wickham GS, Pace NR (1989) Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science 243: 1360-1363.
González AM, Gómez PI, Montoya R (1999) Comparison of PCRRFLP analysis of the ITS region with morphological criteria of various strains of Dunaliella. J. Appl. Phycol. 10: 573-580.
Leonardi PI, Caceres, EJ (1994) Comparative analysis of the fine structure of young and adult individuals of Dunaliella salina with emphasis on the flagellar apparatus. J. Phycol. 30: 642-653.
Loeblich L (1982) Photosynthesis and pigments influenced by light intensity and salinity in the halophile Dunaliella salina (Chlorophyte). J. Mar. Biol. Ass. UK 62: 493-508.
Massyuk NP (1965) Effects of Na, Mg, Cl, and SO4 ions on the growth, reproduction and carotene formation of Dunaliella salina Teod. Ukr. J. Bot. 5: 3-8.
Mendoza H, Jiménez Garcia-Reina, Ramazov Z (1996) Low temperature-induced ?-carotene and fatty acid synthesis, and ultrastructural reorganization of the chloroplast in Dunaliella salina. Eur. J. Phycol. 31: 329-331.
Olmos SJ, Paniagua MJ, Contreras FR (2000) Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene. Lett. Appl. Microbiol. 30: 80-84.
Olsen GJ, Lane DJ, Giovannoni SJ, Pace NR (1986) Microbial ecology and evolution: a ribosomal RNA approach. Annu. Rev. Microbiol. 40: 337-365.
Sambrook KT, Frisch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
Wilcox LW, Lewis A, Fuerst PA, Floyd GL (1992) Group I introns within the nuclear-encoded small-subunit rRNA gene of three green algae. Mol. Biol. Evol. 9: 1103-1118.
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Olmos-Soto, J., Paniagua-Michel, J., Contreras, R. et al. Molecular identification of β-carotene hyper-producing strains of Dunaliella from saline environments using species-specific oligonucleotides. Biotechnology Letters 24, 365–369 (2002). https://doi.org/10.1023/A:1014516920887
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DOI: https://doi.org/10.1023/A:1014516920887