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Effects of NaCl and Proline on Polyphenol Oxidase Activity in Bean Seedlings

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Biologia Plantarum

Abstract

In this work, the effects of NaCl (0, 50, 100, and 150 mM), proline (0, 5 and 10 mM) and NaCl + proline in combinations on activity of polyphenol oxidase (PPO; E.C. 1.10.3.1) and soluble protein content have been investigated in the root, stem and leaf tissues of bean (Phaseolus vulgaris L.) seedlings grown in embryo culture. PPO activities were higher in all the tissues treated with NaCl, proline and NaCl + proline combinations those that of the control tissues. The protein content was very high in tissues exposed to proline and NaCl + proline combination, but NaCl alone decreased protein contents in root and leaf tissues. The results suggest that proline may play a role as an enzyme-stabilizing agent in salt stress.

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References

  • Blum, A., Ebercon A.: Genotypic responses in sorghum to drought stress. III. Free proline accumulation and drought resistance.-Crop Sci. 16: 428-431, 1976.

    Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976.

    Google Scholar 

  • Converso, D.A., Fernandez, M.E., Tomaro, M.L.: Effect of development and salt stress on the activity and expression of wheat peroxidases.-Phyton 61: 147-154, 1997.

    Google Scholar 

  • Demir, Y.: Growth and proline content of germinating wheat genotypes under ultraviolet light.-Turk. J. Bot. 24: 67-70, 2000.

    Google Scholar 

  • Gaurab, G., Sangita, B., Mukherjee, B., Sukumar, G., Gangopadhyay, G., Basu, S., Gupta, S.: Salinity-induced changes on peroxidase in Nicotiana tabacum (var. Jayasri) callus cultures.-Indian J. Plant Physiol. 1: 247-250, 1996.

    Google Scholar 

  • Gomes Filho, E., Sodek, L.: Effect of salinity on ribonuclease activity of Vigna unguiculata cotyledons during germination.-J. Plant Physiol. 132: 307-311, 1988.

    Google Scholar 

  • Hanson, A.D., Nelson, C.E., Eversen, E.H.: Evolution of free proline accumulation as an index of drought resistance using two contrasting barley cultivars.-Crop Sci. 17: 720-726, 1977.

    Google Scholar 

  • Hulme, A.C., Rhodes, M.J.C.: Pome Fruits. The Biochemistry of Their Products.-Academic Press, London 1971.

    Google Scholar 

  • Jennings, P.H., Duffus, C.M.: Effect of giberellic acid on polyphenol oxidase activity in de-embryonated wheat and barley grains.-New Phytol. 78: 383-389, 1977.

    Google Scholar 

  • Kocaçalişkan, I., Demir, Y., Kabar, K.A.: Study on polyphenol oxidase activity during seed germination.-Phyton 35: 37-43, 1995.

    Google Scholar 

  • Kocaçalişkan, I., Özbay, O.: [Changes in enzymatic browning and related factors during development of potato.]-Doga Turk. Biyol. 11: 124-129, 1987. [In Turk.]

    Google Scholar 

  • Lechno, S., Zamski, E., Tel Or, E.: Salt stress-induced responses in cucumber plants.-J. Plant Physiol. 150: 206-211, 1997.

    Google Scholar 

  • Marie, F.R., Jar, P.S., Nikos, B., Jean, P.B., Robert, E.: Effect of salinity on ribonucleolytic activities in glycophytic and halophytic plant species.-J. Plant Physiol. 133: 738-742, 1989.

    Google Scholar 

  • Maxwell, D.P., Bateman, D.F.: Changes in the activity of some oxidases in extracts of rhizoctonia infected bean hypocotyles in relation to lesion maturation.-Phytopathology 57: 132-135, 1967.

    Google Scholar 

  • Miranda, H.M.deL., Loyola-Vargas, V.M.: Glutamate dehydrogenase and glutamine synthase activities in maize under water and salt stress.-Phyton 56: 7-15, 1994.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol Plant. 15: 473-497, 1962.

    Google Scholar 

  • Prisco, J.T., Enear Filho, J. Gomes Filho, E.: Effect of NaCl salinity on cotyledon starch mobilization during germination of Vigna unguiculata L. Walp seeds.-Rev. brasil. Bot. 4: 63-71, 1981.

    Google Scholar 

  • Ramagapol, S.: Salinity stress induced tissue-specific proteins in barley seedlings.-Plant Physiol. 84: 324-331, 1987.

    Google Scholar 

  • Ramagapol, S.: Regulation of protein synthesis in root, shoot and embryonic tissues of germinating barley during salinity stress.-Plant Cell Environ. 11: 501-515, 1988.

    Google Scholar 

  • Sheoran, I.S., Garg, O.P. Effect of salinity on the activities of RNase, DNase, and protease during germination and early seedlings growth of mung bean.-Physiol. Plant. 44: 171-174, 1978.

    Google Scholar 

  • Stewart, G.R., Lee, J.A. The role of proline accumulation in halophytes.-Planta 120: 279-289, 1974.

    Google Scholar 

  • Tiburcio, A.F., Campos, J.L., Figueras, X., Besford, R.T.: Recent advances in the understanding of polyamine functions during plant development.-Plant Growth Regul. 12: 331-340, 1993.

    Google Scholar 

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Demiİr, Y., Kocaçalişkan, İ. Effects of NaCl and Proline on Polyphenol Oxidase Activity in Bean Seedlings. Biologia Plantarum 44, 607–609 (2001). https://doi.org/10.1023/A:1013715425310

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  • DOI: https://doi.org/10.1023/A:1013715425310

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