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Effect of indole-3-acetic acid and kinetin on tuberisation parameters of different cultivars and transgenic lines of potato in vitro

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Abstract

The effect of indole-3-acetic acid or kinetin on the weight and numberof microtubers formed was studied on single node cuttings of sevendifferent potato (Solanum tuberosum L.) cultivars as well astransgenic lines harbouring rolB or rolC genes undercontrol of the patatin class I (B33) promoter. Plants were cultivatedin vitro in the dark on solidified MS medium containing 1 to8% sucrose with or without phytohormones. Most of thenontransformed potato cultivars and transgenic lines responded tohormone application by an increase in tuber yield. Auxin and cytokininacted differently: IAA increased predominantly the tuber size whilekinetin increased the number of tubers. RolC transformantsdisplayed an altered response to sucrose and especially to auxin. Thedegree of phytohormone effect on tuberisation parameters depended onsucrose content of the medium and potato genotype.

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Authors and Affiliations

  1. Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia

    G.A. Romanov;

  2. Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia

    N.P. Aksenova, T.N. Konstantinova & S.A. Golyanovskaya

  3. Max-Planck Institut füur Molekulare Pflanzenphysiologie, D-14476, Golm b. Potsdam, Germany

    J. Kossmann & L. Willmitzer

Authors
  1. G.A. Romanov;
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  2. N.P. Aksenova
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  3. T.N. Konstantinova
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  4. S.A. Golyanovskaya
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  5. J. Kossmann
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  6. L. Willmitzer
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Correspondence to G.A. Romanov;.

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Romanov;, G., Aksenova, N., Konstantinova, T. et al. Effect of indole-3-acetic acid and kinetin on tuberisation parameters of different cultivars and transgenic lines of potato in vitro. Plant Growth Regulation 32, 245–251 (2000). https://doi.org/10.1023/A:1010771510526

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  • DOI: https://doi.org/10.1023/A:1010771510526

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