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Effects of six years atmospheric CO2 enrichment on plant, soil, and soil microbial C of a calcareous grassland

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Abstract

Stimulated plant production and often even larger stimulation of photosynthesis at elevated CO2 raise the possibility of increased C storage in plants and soils. We analysed ecosystem C partitioning and soil C fluxes in calcareous grassland exposed to elevated CO2 for 6 years. At elevated CO2, C pools increased in plants (+23%) and surface litter (+24%), but were not altered in microbes and soil organic matter. Soils were fractionated into particle size and density separates. The amount of low-density macroorganic C, an indicator of particulate soil C inputs from root litter, was not affected by elevated CO2. Incorporation of C fixed during the experiment (Cnew) was tracked by C isotopic analysis of soil fractions which were labelled due to 13C depletion of the commercial CO2 used for atmospheric enrichment. This data constrains estimates of C sequestration (absolute upper bound) and indicates where in soils potentially sequestered C is stored. Cnew entered soils at an initial rate of 210±42 g C m−2 year−1, but only 554±39 g Cnew m−2 were recovered after 6 years due to the low mean residence time of 1.8 years. Previous process-oriented measurements did not indicate increased plant–soil C fluxes at elevated CO2 in the same system (13C kinetics in soil microbes and fine roots after pulse labelling, and minirhizotron observations). Overall experimental evidence suggests that C storage under elevated CO2 occurred only in rapidly turned-over fractions such as plants and detritus, and that potential extra soil C inputs were rapidly re-mineralised. We argue that this inference does not conflict with the observed increases in photosynthetic fixation at elevated CO2, because these are not good predictors of plant growth and soil C fluxes for allometric reasons. C sequestration in this natural system may also be lower than suggested by plant biomass responses to elevated CO2 because C storage may be limited by stabilisation of Cnew in slowly turned-over soil fractions (a prerequisite for long-term storage) rather than by the magnitude of C inputs per se.

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References

  • Amthor J S 1995 Terrestrial higher-plant response to increasing atmospheric [CO2] in relation to the global carbon cycle. Global Change Biol. 1, 243–274.

    Google Scholar 

  • Anderson J P E and Domsch K H 1978 A physiological method for the quantitative measurement of microbial biomass in soils. Soil Biol. Biochem. 10, 215–221.

    Google Scholar 

  • Anderson T-H 1994 Physiological analysis of microbial communities in soil: applications and limitations. In Beyond the Biomass: Compositional and Functional Analysis of Soil Microbial Communities. Eds. K Ritz, J Dighton and K E Giller., pp. 67–76. J Wiley, Chichester.

    Google Scholar 

  • Anderson T-H and Domsch K H 1990 Application of ecophysiological quotients (qCO2 and qD) on microbial biomasses from soils of different cropping histories. Soil Biol. Biochem. 22, 251–255.

    Google Scholar 

  • Arnone J A I and Bohlen P J 1998 Stimulated N2O flux from intact grassland monoliths after two growing seasons under elevated CO2. Oecologia 116, 331–335.

    Google Scholar 

  • Arnone J A, Zaller J G, Spehn E, Niklaus P A, Wells C A and Körner C 2000 Dynamics of roots systems in intact native grasslands: effects of elevated atmospheric CO2. New Phytol. 147, 73–85.

    Google Scholar 

  • Ball A S and Drake B G 1997 Short-term decomposition of litter produced by plants grown in ambient and elevated atmospheric CO2 concentrations. Global Change Biol. 3, 29–35.

    Google Scholar 

  • Benner R, Fogel ML, Sprague E K and Hodson R E 1987 Depletion of 13C in lignin and its implications for stable carbon isotope studies. Nature 329, 708–710.

    Google Scholar 

  • Billes G, Rouhier H and Bottner P 1993 Modifications of the carbon and nitrogen allocations in the plant (Triticum aestivum L.) soil system in response to increased atmospheric CO2 concentration. Plant Soil 157, 215–225.

    Google Scholar 

  • Christensen B T 1992 Physical fractionation of soil and organic matter in primary particle size and density separates. In Advances in Soil Science. (Ed B A Stewart), pp. 1–90. Springer, New York.

    Google Scholar 

  • Cotrufo M F and Gorissen A 1997 Elevated CO2 enhances belowground C allocation in three perennial grass species at different levels of N availability. New Phytol. 137, 421–431.

    Google Scholar 

  • Cotrufo M F and Ineson P 1996 Elevated CO2 reduces field decomposition rates of Betula pendula (Roth.) leaf litter. Oecologia 106, 525–530.

    Google Scholar 

  • Darrah P R 1996 Rhizodeposition under ambient and elevated CO2 levels. Plant Soil 187, 265–275.

    Google Scholar 

  • Drake B G, Muehe M S, Peresta G, Gonzalez Meler M A and Matamala R 1996 Acclimation of photosynthesis, respiration and ecosystem carbon flux of a wetland on Chesapeake Bay, Maryland to elevated atmospheric CO2 concentration. Plant Soil 187, 111–118.

    Google Scholar 

  • Eggers T and Jones T H 2000 You are what you eat...or are you? Trends Ecol. Evolut. 15, 265–266.

    Google Scholar 

  • Elliott E T and Cambardella C A 1991 Physical separation of soil organic matter. Agric. Ecosyst. Environ. 34, 407–419.

    Google Scholar 

  • Eswaran H, Van den Berg E and Reich P 1993 Organic carbon in soils of the world. Soil Sci. Soc. Am. J. 57, 192–194.

    Google Scholar 

  • Fitter A H, Self G K, Wolfenden J, Van Vuuren M M I, Brown T K, Williamson L, Graves J D and Robinson D 1996 Root production and mortality under elevated atmospheric carbon dioxide. Plant Soil 187, 299–306.

    Google Scholar 

  • Gifford R M 1994 The global carbon cycle: a viewpoint on the missing sink. Aust. J. Plant Physiol. 21, 1–15.

    Google Scholar 

  • Gorissen A 1996 Elevated CO2 evokes quantitative and qualitative changes in carbon dynamics in a plant/soil system: mechanisms and implications. Plant Soil 187, 289–298.

    Google Scholar 

  • Ham J M, Owensby C E, Coyne P I and Bremer D J 1995 Fluxes of CO2 and water vapor from a prairie ecosystem exposed to ambient and elevated atmospheric CO2. Agr. Forest Meteorol. 77, 73–93.

    Google Scholar 

  • Hilbert D W, Prudhomme T I and Oechel W C 1987 Response of tussock tundra to elevated carbon dioxide regimes: analysis of ecosystem CO2 flux through nonlinear modeling. Oecologia 72, 466–472.

    Google Scholar 

  • Hirschel G, Körner C and Arnone J A 1997 Will rising atmospheric CO2 affect leaf litter quality and in situ decomposition rates in native plant communities. Oecologia 110, 387–392.

    Google Scholar 

  • Houghton R A 1995 Land-use change and the carbon cycle. Global Change Biol 1, 275–287.

    Google Scholar 

  • Hungate B A, Jackson R B, Field C B and Chapin F S 1996 Detecting changes in soil carbon in CO2 enrichment experiments. Plant Soil 187, 135–145.

    Google Scholar 

  • Hungate B A, Holland E A, Jackson R B, Chapin F S I, Mooney H A and Field C B 1997 The fate of carbon in grasslands under carbon dioxide enrichment. Nature 388, 576–579.

    Google Scholar 

  • Huovinen-Hufschmid C and Körner C 1998 Microscale patterns of plant species distribution, biomass and leaf tissue quality in calcareous grassland. Botan. Helv. 108, 69–83.

    Google Scholar 

  • IGBP 1998 The terrestrial carbon cycle: implications for the Kyoto protocol. Science 280, 1393–1394.

    Google Scholar 

  • Ineson P, Coward P A and Hartwig U A 1998 Soil gas fluxes of N2O, CH4 and CO2 beneath Lolium perenne under elevated CO2: the Swiss free air carbon dioxide enrichment experiment. Plant Soil 198, 89–95.

    Google Scholar 

  • IPCC 1995 Radiative forcing of climate change and an evaluation of the IPCC 1992 IS92 emission scenarios. In (Eds.J T Houghton, L G Meira Filho, B J Hoesung Lee, B A Callander, E H aites, N Harris and K Maskell), pp. 339. Cambridge University Press, Cambridge.

    Google Scholar 

  • Jackson R B, Sala O E, Field C B and Mooney H A 1994 CO2 alters water use, carbon gain, and yield for the dominant species in a natural grassland. Oecologia 98, 257–262.

    Google Scholar 

  • Janssens I A, Kowalski A S, Longdoz B and Ceulemans R 2000 Assessing forest soil CO2 efflux: an in situ comparison of four techniques. Tree Physiol. 20, 23–32.

    Google Scholar 

  • Jensen L S, Mueller T, Tate K R, Ross D J, Magid J and Nielsen N E 1996 Soil surface CO2 flux as an index of soil respiration in situ: A comparison of two chamber methods. Soil Biol. Biochem. 28, 1297–1306.

    Google Scholar 

  • Killham K 1985 A physiological determination of the impact of environmental stress on the activity of microbial biomass. Environ. Pollut. 38, 283–294.

    Google Scholar 

  • Körner C 1996 The response of complex multispecies systems to elevated CO2. In Global Change and Terrestrial Ecosystems. IGBP series no. 2 (Eds. B H Walker and W L Steffen), pp. 20–42. Cambridge University Press, Cambridge.

    Google Scholar 

  • Körner C and Miglietta F 1994 Long term effects of naturally elevated CO2 on mediterranean grassland and forest trees. Oecologia 99, 343–351.

    Google Scholar 

  • Lakhani K H and Satchell J E 1970 Production by Lumbricus terrestris. In Biology and Ecology of Earthworms (Eds. C A Edwards and P J Bohlen), pp. 46–54. Chapman and Hall, London.

    Google Scholar 

  • Lauber W and Körner C 1997In situ stomatal responses to longterm CO2 enrichment in calcareous grassland plants. Acta Oecol. 18, 221–229.

    Google Scholar 

  • Leadley P W, Niklaus P A, Stocker R and Körner C 1997 Screenaided CO2 control (SACC): a middle ground between FACE and open-top chambers. Acta Oecol. 18, 207–219.

    Google Scholar 

  • Leadley P W, Niklaus P A, Stocker R and Körner C 1999 A field study of the effects of elevated CO2 on plant biomass and community structure in a calcareous grassland. Oecologia 118, 39–49.

    Google Scholar 

  • Lekkerkerk L J A, van de Geijn S C and van Veen J A 1990 Effects of elevated atmospheric CO2-levels on the carbon economy of a soil planted with wheat. In Soils and the Greenhouse Effect (EdA F Bouwman), pp. 423–429. John Wiley & Sons

  • Lund C P, Riley W J, Pierce L L and Field C B 1999 The effects of chamber pressurization on soil-surface CO2 efflux and the implications for NEE measurements under elevated CO2. Global Change Biol. 5, 269–281.

    Google Scholar 

  • Luo Y, Chen J L, Reynolds J F, Field C B and Mooney H A 1997 Disproportional increases in photosynthesis and plant biomass in a Californian grassland exposed to elevated CO2: A simulation analysis. Funct. Ecol. 11, 696–704.

    Google Scholar 

  • Luo Y, Jackson R B, Field C B and Mooney H A 1996 Elevated CO2 increases belowground respiration in California grasslands. Oecologia 108, 130–137.

    Google Scholar 

  • Luo Y Q and Reynolds J F 1999 Validity of extrapolating field CO2 experiments to predict carbon sequestration in natural ecosystems. Ecology 80, 1568–1583.

    Google Scholar 

  • Megonigal J P and Schlesinger W H 1997 Enhanced CH4 emissions from a wetland soil exposed to elevated CO2. Biogeochem. 37, 77–88.

    Google Scholar 

  • Neilson R, Hamilton D, Wishart J, Marriott C A, Boag B, Handley L, Scrimgeour C M, McNicol J W and Robinson D 1998 Stable isotope natural abundances of soil, plants and soil invertebrates in an upland pasture. Soil Biol. Biochem. 30, 1773–1782.

    Google Scholar 

  • Newton P C D, Clark H, Bell C C, Glasgow E M, Tate K R, Ross D J, Yeates G W and Saggar S 1995 Plant growth and soil processes in temperate grassland communities at elevated CO2. J. Biogeogr. 22, 235–240.

    Google Scholar 

  • Niklaus P A 1998 Soil microbial effects of elevated CO2 in calcareous grassland. Global Change Biol. 4, 451–458.

    Google Scholar 

  • Niklaus P A, Glöckler E, Siegwolf R and Körner C 2001 Plant– soil carbon fluxes in calcareous grassland under elevated CO2: A combined 13C pulse labeling/soil physical fractionation study. Funct. Ecol. 15, 43–50.

    Google Scholar 

  • Niklaus P A and Körner C 1996 Responses of soil microbiota of a late successional alpine grasslands to long term CO2 enrichment. Plant Soil 184, 219–229.

    Google Scholar 

  • Niklaus P A, Spinnler D and Körner C 1998 Soil moisture dynamics of calcareous grassland under elevated CO2. Oecologia 117, 177–186.

    Google Scholar 

  • Niklaus P A, Stocker R, Körner C and Leadley P W 2000 CO2 flux estimates tend to overestimate ecosystem carbon sequestration at elevated CO2. Funct. Ecol. 14, 546–559.

    Google Scholar 

  • Norby R J, O'Neill E G, Hood G and Luxmoore R J 1987 Carbon allocation, root exudation and mycorrhizal colonization of Pinus echinata seedlings grown under CO2 enrichment. Tree Physiol 3, 203–210.

    Google Scholar 

  • Oades J M 1978 Mucilages at the root surface. Soil Science 29, 1–16.

    Google Scholar 

  • Oades J M 1984 Soil Organic-Matter and Structural Stability-Mechanisms and Implications For Management. Plant Soil 76, 319–337.

    Google Scholar 

  • Oechel W C and Strain B R 1985 Native species responses to increased atmopheric carbon dioxide concentrations. In Direct effects of increasing carbon dioxide on vegetation (Eds. B R Strain and J D Cure), pp. 117–154. U.S. Department of Energy, Washington, D.C.

    Google Scholar 

  • Oechel W C and Strain B R 1986 Native species responses. In Direct effects of carbon dioxide on vegetation (Eds. B R Strain and J D Cure), pp. 117–154. U.S. Department of Energy, Carbon Dioxide Research Division, Washington D.C.

    Google Scholar 

  • Parton W J, Scurlock J M O, Ojima D S, Schimel D S and Hall D O 1995 Impact of climate change on grassland production and soil carbon worldwide. Global Change Biol. 1, 13–22.

    Google Scholar 

  • Ponsard S and Arditi R 2000 What can stable isotopes (delta N-15 and delta C-13) tell about the food web of soil macroinvertebrates? Ecology 81, 852–864.

    Google Scholar 

  • Poorter H, Roumet C and Campbell B D 1996 Interspecific variation in the growth response of plants to elevated CO2: A search for functional types. In Carbon Dioxide, Populations and Communities (Eds. C Körner and F A Bazzaz), pp. 375–412. Academic Press, San Diego.

    Google Scholar 

  • Pregitzer K S, Zak D R, Maziasz J, DeForest J, Curtis P S and Lussenhop J 2000 Interactive effects of atmospheric CO2 and soil-N availability on fine roots of Populus tremuloides. Ecol. Appl. 10, 18–33.

    Google Scholar 

  • Rasmussen P E, Allmaras R R, Rohde C R and Roager N C 1980 Crop residue influences on soil carbon and nitrogen in a wheatfallow system. Soil Sci. Soc. Am. J. 44, 596–600.

    Google Scholar 

  • Rice C W, Garcia F O, Hampton C O and Owensby C E 1994 Soil microbial response in tallgrass prairie to elevated CO2. Plant Soil 165, 67–74.

    Google Scholar 

  • Rillig M C, Wright S F, Allen M F and Field C B 1999 Rise in carbon dioxide changes soil structure. Science 400, 628.

    Google Scholar 

  • Ross D J, Saggar S, Tate K R, Feltham C W and Newton P C D 1996 Elevated CO2 effects on carbon and nitrogen cycling in grass/clover turves of a Psammaquent soil. Plant Soil 182, 185–198.

    Google Scholar 

  • Rouhier H, Billes G, El Kohen A, Mousseau M and Bottner P 1994 Effect of elevated CO2 on carbon and nitrogen distribution within a tree (Castanea sativa Mill.)-soil system. Plant Soil 162, 281–292.

    Google Scholar 

  • Rouhier H, Billes G, Billes L and Bottner P 1996 Carbon fluxes in the rhizosphere of sweet chestnut seedlings (Castanea sativa) grown under two atmospheric CO2 concentrations: 14C Partitioning after pulse labeling. Plant Soil 180, 101–111.

    Google Scholar 

  • Santruckova H, Bird M I and Lloyd J 2000 Microbial processes and carbon-isotope fractionation in tropical and temperate grassland soils. Funct. Ecol. 14, 108–114.

    Google Scholar 

  • Scheu S and Falca M 2000 The soil food web of two beech forests (Fagus sylvatica) of contrasting humus type: stable isotope analysis of a macro-and a mesofauna-dominated community. Oecologia 123, 285–296.

    Google Scholar 

  • Schläpfer M, Zoller H and Körner C 1998 Influences of mowing and grazing on plant species composition in calcareous grassland. Botan. Helv. 108, 57–67.

    Google Scholar 

  • Schlesinger WH 1990 Evidence from chronosequence studies for a low carbon storage potential of soils. Nature 348, 232–234.

    Google Scholar 

  • Stevenson F J and Elliott E T 1989 Methodologies for assessing the quantity and quality of soil organic matter. In Dynamics of Soil Organic Matter in Tropical Ecosystems (Eds. D C Coleman, J M Oades and G Uehara), pp. 173–199. University of Hawaii, Hawaii.

    Google Scholar 

  • Stocker R, Leadley P W and Körner C 1997 Carbon and water fluxes in a calcareous grassland under elevated CO2. Funct. Ecol. 11, 222–230.

    Google Scholar 

  • Thielemann U 1986 Elektrischer Regenwurmfang mit der Oktett-Methode. Pedobiologia 29, 296–302.

    Google Scholar 

  • Van Kessel C, Nitschelm J, Horwath W R, Harris D, Walley F, Luscher A and Hartwig U 2000 Carbon-13 input and turn-over in a pasture soil exposed to long-term elevated atmospheric CO2. Global Change Biol. 6, 123–135.

    Google Scholar 

  • van Veen J A, Liljeroth E and Lekkerkerk L J A 1991 Carbon fluxes in plant–soil systems at elevated atmospheric CO2 levels. Ecol. Appl. 1, 175–181.

    Google Scholar 

  • Van Vuuren M M I, Robinson D, Scrimgeour C M, Raven J A and Fitter A H 2000 Decomposition of C-13-labelled wheat root systems following growth at different CO2 concentrations. Soil Biol. Biochem. 32, 403–413.

    Google Scholar 

  • Vance E D, Brookes P C and Jenkinson D S 1987 An extraction method for measuring soil microbial biomass C. Soil Biol. Biochem. 19, 703–707.

    Google Scholar 

  • Verburg P S J, Gorissen A and Arp W J 1998 Carbon allocation and decomposition of root-derived organic matter in a plant–soil system of Calluna vulgaris as affected by elevated CO2. Soil Biol. Biochem. 30, 1251–1258.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Botany, Schönbeinstrasse 6, CH-4056, Basel, Switzerland

    Pascal A. Niklaus, Monika Wohlfender & Christian Körner

  2. Paul Scherrer Institut, CH-5232, Villigen, Switzerland

    Rolf Siegwolf

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  1. Pascal A. Niklaus
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Niklaus, P.A., Wohlfender, M., Siegwolf, R. et al. Effects of six years atmospheric CO2 enrichment on plant, soil, and soil microbial C of a calcareous grassland. Plant and Soil 233, 189–202 (2001). https://doi.org/10.1023/A:1010389724977

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