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Sago: An Alternative Cheap Gelling Agent for Potato In Vitro Culture

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Biologia Plantarum

Abstract

Sago, a processed (gelatinized) edible starch, was successfully used as a gelling agent in culture medium. The efficacy of sago-gelled (80 g dm−3) medium was studied in ten potato (Solanum tuberosum L.) genotypes during micropropagation and minimal growth conservation. Sago starch provided a firm gelling surface throughout the entire culture period, and fostered optimum plantlet growth in terms of shoot height, number of nodes per plant, number of leaves and fresh mass. No softening of the sago-gelled medium occurred over prolonged (six months) storage. The study showed that sago starch could be used as a substitute to agar in culture medium to substantially reduce the medium cost.

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References

  • Anonymous: The Wealth of India — A Dictionary of Indian Raw Materials and Industrial Products. Vol. 8.-Council of Scientific & Industrial Research, New Delhi 1971.

    Google Scholar 

  • Arnold, S.V., Ericksson, T.: Effect of agar concentration on growth and anatomy of adventitious shoots of Picea abies (L.) Karst.-Plant Cell Tissue Organ Cult. 3: 257–264, 1984.

    Google Scholar 

  • Babbar, S.B., Jain, N.: 'Isubgol' as an alternative gelling agent in plant tissue culture media.-Plant Cell Rep. 17: 318–322, 1998.

    Google Scholar 

  • Gomez, K.A., Gomez, A.A.: Statistical Procedures for Agricultural Research.-John Wiley & Sons, New York 1984.

    Google Scholar 

  • Henderson, W.E., Kinnersley, A.M.: Corn starch as an alternative gelling agent for plant tissue culture.-Plant Cell Tissue Organ Cult. 15: 17–22, 1988.

    Google Scholar 

  • Kohlenbach, H.W., Wernicke, W.: Investigations on the inhibitory effect of agar and the function of active carbon in anther culture.-Z. Pflanzenphysiol. 86: 463–472, 1978.

    Google Scholar 

  • McLachlan, J.: Macroalgae (sea weeds): industrial sources and their utilization.-Plant Soil 89: 137–157, 1985.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Google Scholar 

  • Sarkar, D., Naik, P.S.: Factors affecting minimal growth conservation of potato microplants in vitro.-Euphytica 102: 275–280, 1998.

    Google Scholar 

  • Sarkar, D., Chandra, R., Naik, P.S.: Effect of inoculation density on potato micropropagation.-Plant Cell Tissue Organ Cult. 48: 63–66, 1997.

    Google Scholar 

  • Sorvari, S.: The effect of starch gelatinized nutrient media in barley anther cultures.-Ann. Agr. Fenn. 25: 127–133, 1986a.

    Google Scholar 

  • Sorvari, S.: Differentiation of potato tuber discs in barley starch gelatinized nutrient media.-Ann. Agr. Fenn. 25: 135–138, 1986b.

    Google Scholar 

  • Sorvari, S.: Comparison of anther culture of barley cultivars in barley starch and agar gelatinized media.-Ann. Agr. Fenn. 25: 249–254, 1986c.

    Google Scholar 

  • Thorpe, T.A.: Regulation of organogenesis in vitro.-In: Hughes, K.W., Henke, R., Constantin, M. (ed.): Propagation of Higher Plants Through Tissue Culture: A Bridge Between Research and Application. Pp. 87–101. United States Technical and Information Service, Virginia 1979.

    Google Scholar 

  • Tiwari, S., Rahimbaev, I.: Effect of barley starch in comparison and in combination with agar and agarose on anther culture of Hordeum vulgare L.-Curr. Sci. 62: 430–432, 1992.

    Google Scholar 

  • Zimmerman, R.H., Bhardwaj, S.V., Fordham, I.: Use of starch gelled medium for tissue culture of some fruit crops.-Plant Cell Tissue Organ Cult. 43: 207–213, 1995.

    Google Scholar 

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Naik, P., Sarkar, D. Sago: An Alternative Cheap Gelling Agent for Potato In Vitro Culture. Biologia Plantarum 44, 293–296 (2001). https://doi.org/10.1023/A:1010267929629

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