Abstract
An evaluation was performed of the potential use of AFLP markers to reveal polymorphisms among Lolium perenne plants with different degrees of kinship. Radioactive and fluorescent detection techniques were applied. The use of a fluorescent detection approach contributed greatly to the speed and ease of conducting and interpreting the AFLP patterns. The great discriminative power of AFLP markers and their capacity to represent genetic relationships among ryegrass plants was shown. Despite the high polymorphic value of the AFLP markers, standard statistical tests could not differentiate between two gene pools derived from different breeding programmes. It proved also impossible to correlate fodder and turf phenotypes with AFLP distance data. A very important point revealed by our data is the high degree of genetic diversity within commercial ryegrass varieties. Our findings are relevant to any outcrossing crop with a breeding strategy based on the production of synthetic populations.
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Baril CP, Verhaegen D, Vigneron P, Bouvet JM, Kremer A: Structure of the specific combining ability between two species of Eucalyptus. I. RAPD data. Theor Appl Genet 94: 796-803 (1997).
Becker J, Vos P, Kuiper M, Salamini F, Heun M: Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet 249: 65-73 (1995).
Bernardo R: Estimation of coefficient of coancestry using molecular markers in maize. Theor Appl Genet 85: 1055-1062 (1993).
Booy G, van Dreven F, Steverink-Raben A: Identification of ryegrass varieties (Lolium spp.) using allele frequencies of the PGI-2 and ACP-1 isozyme systems. Plant Var Seeds 6: 179-196 (1993).
Charmet G, Balfourier F: Isozyme variation and species relationships in the genus Lolium L. (ryegrasses, Gramineaceae). Theor Appl Genet 87: 641-649 (1994).
Dillmann C, Bar-Hen A, Guérin D, Charcosset A, Murigneux A: Comparison of RFLP andmorphological distances between maize Zea mays L. inbred lines. Consequences for germplasm protection purposes. Theor Appl Genet 95: 92-102 (1997).
Jaccard P: Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223-270 (1908).
Hill MO: DECORANA: a Fortran program for detrended correspondence analysis and reciprocal averaging. Cornell University, Ithaca NY (1979).
Hongtrakul V, Huestis GM, Knapp SJ: Amplified fragment length polymorphisms as a tool for DNA fingerprinting sun-flower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 95: 400-407 (1997).
Lanza LLB, de Souza CLJ, Ottoboni LMM, Vieira MLC, de Souza AP: Genetic distance of inbred lines and prediction of maize single-cross performance using RAPD markers. Theor Appl Genet 94: 1023-1030 (1997).
Law JR, Donini P, Koebner RMD, Reeves JC, Cooke RJ: Statistical methods for assessing and interpreting genetic diversity. In: Advances in Biometrical Genetics. Proceedings of the 10th meeting of the Eucarpia Section, Biometrics in Plant Breeding, Poznan (1997).
Norusis NJ: SPSS for windows: base system. User's Guide, Release 6.0 (1993).
Paglia G, Morgante M: PCR-based multiplex DNA fingerprinting techniques for the analysis of conifer genomes. Mol Breed 4: 173-177 (1998).
Paran I, Aftergoot E, Shifriss C: Variation in Capsicum annuum revealed by RAPD and AFLP markers. Euphytica 99: 167-173 (1998).
Perkin Elmer, Applied Biosystems: ABI Prism GeneScan Analysis Software. User's manual (1996a).
Perkin Elmer, Applied Biosystems: ABI Prism Genotyper 2.0. User's manual, (1996).
Reamon-Buttner SM, Schoudelmaer J, Jung C: AFLP markers linked to the sex locus in Asparagus officinalis L. Mol Breed 4: 91-98 (1998).
Roldán-Ruiz I, Van Laecke K, De Riek J, Calsyn E, Depicker A, Van Bockstaele E, Beerepoot L., De Loose M: The use of molecular markers for assessing genetic conformity between varieties of ryegrass. Med Fac Landbouw Univ Gent. 62/4a (1997).
Roldán-Ruiz I, Depicker A, Dendauw J, Van Bockstale E, De Loose M: Comparison of different methods to score radioactive AFLP gels. Proceedings of the 6th Plant and Animal Genome Meeting, San Diego, CA (1998).
ter Braak CJF: CANOCO: a Fortran program for canonical community ordination by [partial] [detrended] [canonical] correspondence analysis, principal components analysis and redundancy analysis (version 2.1.). Agricultural Mathematics Group, Wageningen, Netherlands (1978).
Tsegaye S, Tesemma T, Belay G: Relationships among tetraploid wheat (Triticum turgidum L.) landrace populations revealed by isozyme markers and agronomic traits. Theor appl Genet 93: 600-605 (1996).
Van Bockstaele E: Aspects of breeding perennial ryegrass (Lolium perenne). Plant Sci 35: 690-694 (1998).
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M: AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407-4414 (1995).
Weising K, Beyermann B, Ramser J, Kahl G: Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12: 159-169 (1991).
Zabeau M, Vos P: European Patent Application, publication EP 0534858 (1993).
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Roldán-Ruiz, I., Dendauw, J., Van Bockstaele, E. et al. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding 6, 125–134 (2000). https://doi.org/10.1023/A:1009680614564
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DOI: https://doi.org/10.1023/A:1009680614564