Plant Molecular Biology

, Volume 41, Issue 5, pp 645–655

A conserved His-Asp signal response regulator-like gene in Heterosigma akashiwo chloroplasts

  • Michael A. Jacobs
  • Laurie Connell
  • Rose Ann Cattolico
Article

DOI: 10.1023/A:1006394925182

Cite this article as:
Jacobs, M.A., Connell, L. & Cattolico, R.A. Plant Mol Biol (1999) 41: 645. doi:10.1023/A:1006394925182

Abstract

Regulation of gene expression in plastids may involve molecular components conserved from cyanobacteria-like ancestors. Among prokaryotes, genes are commonly regulated at the transcriptional level by `two-component' or `His-Asp' signal transducers, consisting of a `sensor kinase', which autophosphorylates at a conserved histidine residue, and a cognate response regulator, which is phosphorylated by the sensor kinase at a conserved aspartate residue. A putative His-Asp response regulator gene (trg1: transcriptional regulatory gene 1) has been identified in the estuarine raphidophytic alga Heterosigma akashiwo. The chloroplast-encoded trg1 is 693 bp in length, contains no introns, and yields a conceptual translation product of 231 amino acids, with a predicted mass of 27 kDa. Homology searches suggest that Heterosigma trg1 has an ompR-like identity within the DNA-binding His-Asp family of response regulators. trg1 contains both the phosphorylation and DNA-binding domains which are present in prokaryote response regulators. Quantitative competitive RT-PCR showed that Heterosigma trg1 is expressed at low levels (5 μg per g total RNA). In contrast, psbA (a photosystem II component) transcript is abundant (60 mg per g total RNA). Cell cycle analysis showed that psbA abundance oscillates in response to light but trg1 mRNA levels are invariant. We hypothesize that a His-Asp phosphorelay mechanism may affect chloroplast genome transcription in a manner similar to bacterial signal transduction pathways in which `sensor kinase' and cognate `response regulator' proteins interact.

chloroplastHeterosigmaHis-Aspresponse regulatorsignal transduction

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Michael A. Jacobs
    • 1
  • Laurie Connell
    • 1
  • Rose Ann Cattolico
    • 2
  1. 1.Department of BotanyUniversity of WashingtonSeattleUSA
  2. 2.Departments of Botany and OceanographyUniversity of WashingtonSeattleUSA