Plant Molecular Biology

, Volume 37, Issue 6, pp 911–919

Distribution patterns of 104 kDa stress-associated protein in rice

  • Sneh Lata Singla
  • Ashwani Pareek
  • Anil Kumar Kush
  • Anil Grover
Article

DOI: 10.1023/A:1006099715375

Cite this article as:
Lata Singla, S., Pareek, A., Kumar Kush, A. et al. Plant Mol Biol (1998) 37: 911. doi:10.1023/A:1006099715375

Abstract

A 104 kDa protein (SAP 104) accumulates in rice seedlings in response to several abiotic stress conditions and immunological homologues of rice SAP 104 have been detected in several monocot and dicot species, as also Neurospora crassa, a fungus. In this report, we show that the amino acid sequence of a tryptic peptide generated from purified SAP 104 bears significant homology with an ATP-binding domain of the HSP 100 family proteins of Arabidopsis thaliana and Glycine max. It is further shown that differential uninduced and induced (by high-temperature stress) levels of this protein are accumulated in various organs of the mature rice plant grown under field conditions. Significant uninduced levels of this protein were in particular found in developing and mature rice grains. Seeds/grains of several other plant genera (i.e. Triticum aestivum, Zea mays, Brassica juncea) were also found to contain high uninduced levels of SAP 104. Importantly, the levels of uninduced SAP 104 in rice grains were found to decline during the seed germination phase: after two days of germination, this protein was undetectable in tissues representing pooled sample of seeds and just-emerged seedlings. Tissue print-immunoblotting analysis has indicated that in seeds high levels of this protein are specifically present in the embryo portion.

developmental regulationheat shock proteinOryza sativa L.OsHSP 110SAP 104seed germinationseed localization

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Sneh Lata Singla
    • 1
  • Ashwani Pareek
    • 1
  • Anil Kumar Kush
    • 2
  • Anil Grover
    • 1
  1. 1.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew Delhi-India
  2. 2.Institute of Molecular and Cell BiologyNational University of SingaporeLower Kent Ridge, SingaporeSingapore;
  3. 3.Department of BiologyUniversity of North Carolina, Coker HallChapel HillUSA
  4. 4.Indo-American Hybrid Seeds, 17th Cross, 2nd ‘A’ MainBangaloreIndia