Abstract
Cell suspension cultures were initiated from gametophyte-derived callus of the fern Platycerium coronarium. Two distinct types of callus masses, distinguished by their colouration, were obtained when the cells from suspension culture were plated on semisolid Murashige and Skoog (MS) medium containing 10 μM kinetin. The two types of callus masses had distinct morphogenetic capacities despite their common origin. Morphogenesis into either gametophytes or sporophytes occurred when these callus masses were cultured on phytohormone-free MS medium depending on the type of callus used. The dark-green gametophytic callus showed a faster rate of growth and morphogenesis as compared to the pale-green sporophytic callus. Total chlorophyll content and autofluorescence and size of chloroplasts of the sporophytic callus and cell suspension cultures were lower than that of the gametophytic callus. Observations from confocal laser scanning microscopy were in agreement with the physiological parameters measured. The availability of cell cultures of the same ploidy level, but with two distinct pathways of development will be useful for comparative studies of developmental plasticity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barba R & Nickell LG (1969) Nutrition and organ differentiation in tissue cultures of sugarcane, a monocotyledon. Planta 89: 299–302
Byrne TE & Caponetti JD (1992a) Morphogenesis in three cultivars of Boston fern. II. Callus production from stolon tips and plantlet differentiation from callus. Am. Fern J. 82: 1–11
Byrne TE & Caponetti JD (1992b) Morphogenesis in three cultivars of Boston fern. III. Callus production and plantlet differentiation from cell suspensions. Am. Fern J. 82: 12–22
Caponetti JD, Whitten M & Beck MJ (1982) Axenic culture and induction of callus and sporophytes of the Appalachian Vittaria gametophyte. Am. Fern J. 72: 36–40
Cooke R C (1979) Homogenisation as an aid in tissue culture propagation of Platycerium and Davallia. HortSci. 14: 21–22
Gautheret RJ (1966) Factors affecting differentiation of plant tissues in vitro. In: Beermann (ed) Cell Differentiation and Morphogenesis. (pp 55–95). North-Holland publishing Co., Amsterdam
Hennen GR & Sheehan TJ (1978) In vitro propagation of Platycerium stemaria (Beauvois) Desv. HortSci. 13: 245
Hickok L G, Warne TR & Slocum MK (1987) Ceratopteris richardii: Applications for experimental plant biology. Am. J. Bot. 59: 458–465
Higuchi H, Anaki W & Suzuki S (1987) In vitro propagation of Nephrolepis cordifolia Presl. Sci. Horticult. 32: 105–113
Howard J, Shannon L, Oki L & Murashige T (1977) Soybean agglutinin-a mitogen for soybean callus cells. Exp. Cell Res. 107: 448–450
Kwa SH, Wee YC, Lim TM & Kumar PP (1995a) IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro. Plant Cell Rep. 14: 598–602
Kwa SH, Wee YC, Lim TM & Kumar PP (1995b) Establishment and physiological analyses of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) Desv. under CO2 enrichment. J. Exp. Bot. 46: 1535–1542
Kumar PP, Bender L & Thorpe TA (1988) Activities of ribulose bisphosphate carboxylase and phosphoenolpyruvate carboxylase and 14C-bicarbonate fixation during in vitro culture of Pinus radiata cotyledons. Plant Physiol. 87: 675–679
Mahabale TS & Patankar TB (1980) In vitro morphogenetic studies on the gametophytic callus of Drynaria quercifolia. Bio-Vigyanam 6: 11–14
Melan MA & Whittier DP (1990) Effects of inorganic nitrogen sources on spore germination and gametophyte growth in Botrychium dissectum. Plant Cell Envir. 13: 477–482
Miller JH & Wagner PM (1987) Co-requirement of calcium and potassium in the germination of spores of the fern Onoclea sensibilis. Am. J. Bot. 74: 1585–1589
Muir WH, Hildebrandt AC & Riker AJ (1958) The preparation, isolation and growth in culture of single cells from higher plants. Am. J. Bot. 45: 589–597
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 5: 473–497
Nester JE & Coolbaugh RC (1986) Factors influencing spore germination and early gametophyte development in Anemia mexicana and Anemia phyllitidis. Plant Physiol. 82: 230–235
Padhya MA & Mehta AR (1982) Propagation of fern (Nephrolepis) through tissue culture. Plant Cell Rep. 1: 261–263
Steward FC, Mapes MO & Mears K (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am. J. Bot. 45: 705–708
Syono K (1965) Changes in organ forming capacity of carrot root calluses during subculture. Plant Cell Physiol. 6: 403–419
Thorpe TA (1988) In vitro Somatic Embryogenesis. ISI Atlas of Science: Animal and Plant Sciences. (pp 81–88)
Wochok ZS & Sussex IM (1976) Redetermination of cultured root tips to leafy shoots in Selaginella willdenovii. Plant Sci. Lett. 6: 185–192
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kwa, SH., Wee, YC., Lim, TM. et al. Morphogenetic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium. Plant Cell, Tissue and Organ Culture 48, 37–44 (1997). https://doi.org/10.1023/A:1005756822370
Issue Date:
DOI: https://doi.org/10.1023/A:1005756822370