Skip to main content
Log in

Culture conditions for a new phytase-producing fungus

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Extracellular phytase produced by Aspergillus sp. 5990 showed a 5-fold higher activity in liquid culture when compared with cultures of Aspergillus ficuum NRRL 3135. The optimum fermentation conditions were determined to be 35 °C, neutral pH, and 4 days incubation. The phytase had a higher optimum temperature for its activity than the commercial enzyme, Natuphos, from Aspergillus ficuum NRRL 3135.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chelius MK, Wodzinski RJ (1994) Strain improvement of Aspergillus niger for phytase production. Appl. Microbiol. Biotechnol. 41: 79–83.

    Google Scholar 

  • Cheryan M (1980) Phytic acid interactions in food systems. CRC Crit. Rev. Food Sci. Nutr. 13: 297–335.

    Google Scholar 

  • Dvorakova J, Volfova O, Kopecky J (1997) Characterization of phytase produced from Aspergillus niger. Folia Microbiol. 42: 349–352.

    Google Scholar 

  • Gargova S, Roshkova Z, Vancheva G (1997) Screening of fungi for phytase production. Biotechnol. Tech. 11: 221–224.

    Google Scholar 

  • Greiner R, Haller E, Konietzny U, Jany K-D (1997) Purification and characterization of a phytase from Klebsiella terrigena. Arch. Biochem. Biophys. 341: 201–206.

    Google Scholar 

  • Howson SJ, Davis RP (1983) Production of phytate-hydrolyzing enzyme by some fungi. Enzyme Microbiol. Technol. 5: 377–382.

    Google Scholar 

  • Kim YO, Kim HK, Bae KS, Yu JH, Oh TK (1998) Purification and properties of a thermostable phytase from Bacillus sp. DS11. Enzyme Microbiol. Technol. 22: 2–7.

    Google Scholar 

  • Lambrechts C, Boze H, Segueilba L, Moulin G, Galzy P (1992) Utilization of phytate by some yeasts. Biotechnol. Lett. 14: 61–66.

    Google Scholar 

  • Pasamontes L, Haiker M, Wyss M, Tessier M, van Loon APGM (1997) Gene cloning, purification, and characterization of a heat stable phytase from the fungus Aspergillus fumigatus. Appl. Environ. Microbiol. 63: 1696–1700.

    Google Scholar 

  • Peddington CS, Houston CS, Paloheimo M, Cantrell M, Miettinen-Oinonen A, Nevalainen H, Rambosek (1993) The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori. Gene 133: 55–62.

    Google Scholar 

  • Shieh TR, Ware JH (1968) Survey of microorganisms for the production of extracellular phytase. Appl. Microbiol. 16: 1348–1351.

    Google Scholar 

  • Shimizu M (1993) Purification and characterization of phytase from Bacillus subtilis (natto) N-77. Biosci. Biotech. Biochem. 56: 1266–1269.

    Google Scholar 

  • Ullah AHJ, Gibson DM (1987) Extracellular phytase (E.C. 3.1.3.8.) from Aspergillus ficuum NRRL 3135: purification and characterization. Prep. Biochem. 17: 63–91.

    Google Scholar 

  • Utt EA (1987) The strain improvement of Aspergillus ficuum NRRL 3135. MSc Thesis. Florida, USA: University of Central Florida.

    Google Scholar 

  • Wyss M, Brugger R, Kronenberger A, Remy R, Fimbel R, Oesterhelt G, Lehmann M, van Loon APGM (1999) Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl. Environ. Microbiol. 65: 367–373.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, DS., Godber, J.S. & Kim, HR. Culture conditions for a new phytase-producing fungus. Biotechnology Letters 21, 1077–1081 (1999). https://doi.org/10.1023/A:1005696829168

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005696829168

Navigation